Research Portal

Isolation and characterisation of smallminded, a Drosophila gene encoding a new member of the Cdc48p/VCP subfamily of AAA proteins

Research output: Contribution to journal › Article › peer-review

Standard Standard

Isolation and characterisation of smallminded, a Drosophila gene encoding a new member of the Cdc48p/VCP subfamily of AAA proteins. / Long, Antony R; Yang, Mingyao; Kaiser, Kim et al.
In: Gene, Vol. 208, No. 2, 27.02.1998, p. 191-9.

Research output: Contribution to journal › Article › peer-review

HarvardHarvard

Long, AR, Yang, M, Kaiser, K & Shepherd, D 1998, 'Isolation and characterisation of smallminded, a Drosophila gene encoding a new member of the Cdc48p/VCP subfamily of AAA proteins', Gene, vol. 208, no. 2, pp. 191-9. https://doi.org/10.1016/S0378-1119(97)00652-5

APA

Long, A. R., Yang, M., Kaiser, K., & Shepherd, D. (1998). Isolation and characterisation of smallminded, a Drosophila gene encoding a new member of the Cdc48p/VCP subfamily of AAA proteins. Gene, 208(2), 191-9. https://doi.org/10.1016/S0378-1119(97)00652-5

CBE

Long AR, Yang M, Kaiser K, Shepherd D. 1998. Isolation and characterisation of smallminded, a Drosophila gene encoding a new member of the Cdc48p/VCP subfamily of AAA proteins. Gene. 208(2):191-9. https://doi.org/10.1016/S0378-1119(97)00652-5

MLA

Long, Antony R et al. "Isolation and characterisation of smallminded, a Drosophila gene encoding a new member of the Cdc48p/VCP subfamily of AAA proteins". Gene. 1998, 208(2). 191-9. https://doi.org/10.1016/S0378-1119(97)00652-5

VancouverVancouver

Long AR, Yang M, Kaiser K, Shepherd D. Isolation and characterisation of smallminded, a Drosophila gene encoding a new member of the Cdc48p/VCP subfamily of AAA proteins. Gene. 1998 Feb 27;208(2):191-9. doi: 10.1016/S0378-1119(97)00652-5

Author

Long, Antony R ; Yang, Mingyao ; Kaiser, Kim et al. / Isolation and characterisation of smallminded, a Drosophila gene encoding a new member of the Cdc48p/VCP subfamily of AAA proteins. In: Gene. 1998 ; Vol. 208, No. 2. pp. 191-9.

RIS

TY - JOUR

T1 - Isolation and characterisation of smallminded, a Drosophila gene encoding a new member of the Cdc48p/VCP subfamily of AAA proteins

AU - Long, Antony R

AU - Yang, Mingyao

AU - Kaiser, Kim

AU - Shepherd, David

PY - 1998/2/27

Y1 - 1998/2/27

N2 - Smallminded (smid) encodes a new member of the cdc48p/VCP subfamily of AAA proteins in Drosophila. The gene was isolated by plasmid rescue from a GAL4 enhancer trap line which shows reporter gene expression in neuroblasts, imaginal disks and a subset of sensory neurons. Larvae homozygous for the insert arrest development as second instar larvae and die without pupating. The most obvious defect in these larvae is a significantly reduced CNS, hence the naming of the gene as smallminded. The deduced amino acid sequence of smid contains a tandem duplication of the AAA nucleotide binding domain characteristic of the cdc48p/VCP subfamily. Overall, smid shares 33% identical residues with its closest relative, yeast L0919-chrXII and 26-29% with other members of the cdc48p/VCP subfamily. The most highly conserved regions of the predicted protein structure are found in and around the nucleotide binding domains. The gene is expressed at all developmental stages.

AB - Smallminded (smid) encodes a new member of the cdc48p/VCP subfamily of AAA proteins in Drosophila. The gene was isolated by plasmid rescue from a GAL4 enhancer trap line which shows reporter gene expression in neuroblasts, imaginal disks and a subset of sensory neurons. Larvae homozygous for the insert arrest development as second instar larvae and die without pupating. The most obvious defect in these larvae is a significantly reduced CNS, hence the naming of the gene as smallminded. The deduced amino acid sequence of smid contains a tandem duplication of the AAA nucleotide binding domain characteristic of the cdc48p/VCP subfamily. Overall, smid shares 33% identical residues with its closest relative, yeast L0919-chrXII and 26-29% with other members of the cdc48p/VCP subfamily. The most highly conserved regions of the predicted protein structure are found in and around the nucleotide binding domains. The gene is expressed at all developmental stages.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Brain/anatomy & histology

KW - Cell Cycle Proteins/biosynthesis

KW - Cloning, Molecular

KW - Drosophila/genetics

KW - Drosophila Proteins

KW - Genes, Insect

KW - Genes, Reporter

KW - Heterozygote

KW - Homozygote

KW - Larva

KW - Molecular Sequence Data

KW - Neurons, Afferent/metabolism

KW - Recombinant Fusion Proteins/biosynthesis

KW - Sequence Alignment

KW - Sequence Homology, Amino Acid

U2 - 10.1016/S0378-1119(97)00652-5

DO - 10.1016/S0378-1119(97)00652-5

M3 - Article

C2 - 9524263

VL - 208

SP - 191

EP - 199

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2

ER -