Isolation of new Klebsiella pneumoniae phage PSKP16

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

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Dangosydd eitem ddigidol (DOI)

  • Sara Sadeqi
    Qazvin University of Medical Sciences
  • Slawomir Michniewski
    University of Warwick
  • Farhad Nikkhahi
    Qazvin University of Medical Sciences
  • Eleanor Jameson
    Norwegian University of Life Sciences, Department of Mathematical Science and TechnologyUniversity of WarwickMMU
  • Seyed Mahmoud Amin Marashi
    Qazvin University of Medical Sciences

BACKGROUND AND OBJECTIVES: Klebsiella pneumoniae is a clinically relevant opportunistic pathogen belonging to the Enterobacteriaceae family. It is in the top three bacteria associated with antimicrobial resistance deaths globally, and one of the most dangerous bacteria causing nosocomial infections. Phage therapy offers a potential option for the treatment of drug-resistant bacterial infections.

MATERIALS AND METHODS: Phage PSKP16 was isolated against K. pneumoniae, capsular type K2 (isolated from a wound infection). PSKP16 is a new lytic phage with a Siphovirus-like morphology.

RESULTS: PSKP16 is a linear double stranded DNA phage with a GC content of 50% and genome size of 46,712 bp, for which we predicted 67 ORFs. PSKP16 belongs to the genus Webervirus and shows high evolutionary proximity to Klebsiella phages JY917, Sushi, and B1.

CONCLUSION: Phage isolation is fast, cheap and efficient, but it requires time and characterization (which adds expense) to ensure that the isolated phages do not pose a health risk, which is essential to safely use phage therapy to treat life-threatening bacterial infections.

Iaith wreiddiolSaesneg
Tudalennau (o-i)62-68
Nifer y tudalennau7
CyfnodolynIranian journal of microbiology
Cyfrol15
Rhif y cyfnodolyn1
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 1 Chwef 2023
Gweld graff cysylltiadau