TY - JOUR

T1 - Isolation of new Klebsiella pneumoniae phage PSKP16

AU - Sadeqi, Sara

AU - Michniewski, Slawomir

AU - Nikkhahi, Farhad

AU - Jameson, Eleanor

AU - Marashi, Seyed Mahmoud Amin

N1 - Copyright © 2023 The Authors. Published by Tehran University of Medical Sciences.

PY - 2023/2/1

Y1 - 2023/2/1

N2 - BACKGROUND AND OBJECTIVES: Klebsiella pneumoniae is a clinically relevant opportunistic pathogen belonging to the Enterobacteriaceae family. It is in the top three bacteria associated with antimicrobial resistance deaths globally, and one of the most dangerous bacteria causing nosocomial infections. Phage therapy offers a potential option for the treatment of drug-resistant bacterial infections.MATERIALS AND METHODS: Phage PSKP16 was isolated against K. pneumoniae, capsular type K2 (isolated from a wound infection). PSKP16 is a new lytic phage with a Siphovirus-like morphology.RESULTS: PSKP16 is a linear double stranded DNA phage with a GC content of 50% and genome size of 46,712 bp, for which we predicted 67 ORFs. PSKP16 belongs to the genus Webervirus and shows high evolutionary proximity to Klebsiella phages JY917, Sushi, and B1.CONCLUSION: Phage isolation is fast, cheap and efficient, but it requires time and characterization (which adds expense) to ensure that the isolated phages do not pose a health risk, which is essential to safely use phage therapy to treat life-threatening bacterial infections.

AB - BACKGROUND AND OBJECTIVES: Klebsiella pneumoniae is a clinically relevant opportunistic pathogen belonging to the Enterobacteriaceae family. It is in the top three bacteria associated with antimicrobial resistance deaths globally, and one of the most dangerous bacteria causing nosocomial infections. Phage therapy offers a potential option for the treatment of drug-resistant bacterial infections.MATERIALS AND METHODS: Phage PSKP16 was isolated against K. pneumoniae, capsular type K2 (isolated from a wound infection). PSKP16 is a new lytic phage with a Siphovirus-like morphology.RESULTS: PSKP16 is a linear double stranded DNA phage with a GC content of 50% and genome size of 46,712 bp, for which we predicted 67 ORFs. PSKP16 belongs to the genus Webervirus and shows high evolutionary proximity to Klebsiella phages JY917, Sushi, and B1.CONCLUSION: Phage isolation is fast, cheap and efficient, but it requires time and characterization (which adds expense) to ensure that the isolated phages do not pose a health risk, which is essential to safely use phage therapy to treat life-threatening bacterial infections.

U2 - 10.18502/ijm.v15i1.11919

DO - 10.18502/ijm.v15i1.11919

M3 - Article

C2 - 37069906

VL - 15

SP - 62

EP - 68

JO - Iranian journal of microbiology

JF - Iranian journal of microbiology

SN - 2008-3289

IS - 1

ER -