Prenylated FMN: biosynthesis, purification, and Fdc1 activation

Allbwn ymchwil: Pennod mewn Llyfr/Adroddiad/Trafodion CynhadleddPennod

Fersiynau electronig

Dangosydd eitem ddigidol (DOI)

  • Anna N. Khusnutdinova
    University of Toronto, Canada
  • Johnny Xiao
    University of Toronto, Canada
  • Po-Hsiang Wang
    University of Toronto, Canada
  • Khorcheska Batyrova
    University of Toronto, Canada
  • Robert Flick
    University of Toronto, Canada
  • Elizabeth Edwards
    University of Toronto, Canada
  • Alexander Yakunin
Prenylated flavin mononucleotide (prFMN) is a recently discovered flavin cofactor produced by the UbiX family of FMN prenyltransferases, and is required for the activity of UbiD-like reversible decarboxylases. The latter enzymes are known to be involved in ubiquinone biosynthesis and biotransformation of lignin, aromatic compounds, and unsaturated aliphatic acids. However, exploration of uncharacterized UbiD proteins for biotechnological applications is hindered by our limited knowledge about the biochemistry of prFMN and prFMN-dependent enzymes. Here, we describe experimental protocols and considerations for the biosynthesis of prFMN in vivo and in vitro, in addition to cofactor extraction and application for activation of UbiD proteins.
Iaith wreiddiolSaesneg
TeitlNew Approaches for Flavin Catalysis
GolygyddionBruce A. Palfey
CyhoeddwrElsevier
Pennod18
Tudalennau469-488
Cyfrol620
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 2019

Cyfres gyhoeddiadau

EnwMethods in Enzymology
CyhoeddwrElsevier
ISSN (Argraffiad)0076-6879
Gweld graff cysylltiadau