Prenylated FMN: biosynthesis, purification, and Fdc1 activation
Allbwn ymchwil: Pennod mewn Llyfr/Adroddiad/Trafodion Cynhadledd › Pennod › adolygiad gan gymheiriaid
Fersiynau electronig
Dangosydd eitem ddigidol (DOI)
Prenylated flavin mononucleotide (prFMN) is a recently discovered flavin cofactor produced by the UbiX family of FMN prenyltransferases, and is required for the activity of UbiD-like reversible decarboxylases. The latter enzymes are known to be involved in ubiquinone biosynthesis and biotransformation of lignin, aromatic compounds, and unsaturated aliphatic acids. However, exploration of uncharacterized UbiD proteins for biotechnological applications is hindered by our limited knowledge about the biochemistry of prFMN and prFMN-dependent enzymes. Here, we describe experimental protocols and considerations for the biosynthesis of prFMN in vivo and in vitro, in addition to cofactor extraction and application for activation of UbiD proteins.
| Iaith wreiddiol | Saesneg |
|---|---|
| Teitl | New Approaches for Flavin Catalysis |
| Golygyddion | Bruce A. Palfey |
| Cyhoeddwr | Elsevier |
| Pennod | 18 |
| Tudalennau | 469-488 |
| Cyfrol | 620 |
| Dynodwyr Gwrthrych Digidol (DOIs) | |
| Statws | Cyhoeddwyd - 2019 |
Cyfres gyhoeddiadau
| Enw | Methods in Enzymology |
|---|---|
| Cyhoeddwr | Elsevier |
| ISSN (Argraffiad) | 0076-6879 |