TY - CHAP

T1 - Prenylated FMN: biosynthesis, purification, and Fdc1 activation

AU - Khusnutdinova, Anna N.

AU - Xiao, Johnny

AU - Wang, Po-Hsiang

AU - Batyrova, Khorcheska

AU - Flick, Robert

AU - Edwards, Elizabeth

AU - Yakunin, Alexander

PY - 2019

Y1 - 2019

N2 - Prenylated flavin mononucleotide (prFMN) is a recently discovered flavin cofactor produced by the UbiX family of FMN prenyltransferases, and is required for the activity of UbiD-like reversible decarboxylases. The latter enzymes are known to be involved in ubiquinone biosynthesis and biotransformation of lignin, aromatic compounds, and unsaturated aliphatic acids. However, exploration of uncharacterized UbiD proteins for biotechnological applications is hindered by our limited knowledge about the biochemistry of prFMN and prFMN-dependent enzymes. Here, we describe experimental protocols and considerations for the biosynthesis of prFMN in vivo and in vitro, in addition to cofactor extraction and application for activation of UbiD proteins.

AB - Prenylated flavin mononucleotide (prFMN) is a recently discovered flavin cofactor produced by the UbiX family of FMN prenyltransferases, and is required for the activity of UbiD-like reversible decarboxylases. The latter enzymes are known to be involved in ubiquinone biosynthesis and biotransformation of lignin, aromatic compounds, and unsaturated aliphatic acids. However, exploration of uncharacterized UbiD proteins for biotechnological applications is hindered by our limited knowledge about the biochemistry of prFMN and prFMN-dependent enzymes. Here, we describe experimental protocols and considerations for the biosynthesis of prFMN in vivo and in vitro, in addition to cofactor extraction and application for activation of UbiD proteins.

U2 - 10.1016/bs.mie.2019.03.021

DO - 10.1016/bs.mie.2019.03.021

M3 - Chapter

VL - 620

T3 - Methods in Enzymology

SP - 469

EP - 488

BT - New Approaches for Flavin Catalysis

A2 - Palfey, Bruce A.

PB - Elsevier

ER -