Riding the wave of genomics to investigate aquatic coliphage diversity and activity
Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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Yn: Environmental Microbiology, Cyfrol 21, Rhif 6, 01.06.2019, t. 2112-2128.
Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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T1 - Riding the wave of genomics to investigate aquatic coliphage diversity and activity
AU - Michniewski, Slawomir
AU - Redgwell, Tamsin
AU - Grigonyte, Aurelija
AU - Rihtman, Branko
AU - Aguilo-Ferretjans, Maria
AU - Christie-Oleza, Joseph
AU - Jameson, Eleanor
AU - Scanlan, David J
AU - Millard, Andrew D
N1 - © 2019 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Bacteriophages infecting Escherichia coli (coliphages) have been used as a proxy for faecal matter and water quality from a variety of environments. However, the diversity of coliphages that is present in seawater remains largely unknown, with previous studies largely focusing on morphological diversity. Here, we isolated and characterized coliphages from three coastal locations in the United Kingdom and Poland. Comparative genomics and phylogenetic analysis of phage isolates facilitated the identification of putative new species within the genera Rb69virus and T5virus and a putative new genus within the subfamily Tunavirinae. Furthermore, genomic and proteomic analysis combined with host range analysis allowed the identification of a putative tail fibre that is likely responsible for the observed differences in host range of phages vB_Eco_mar003J3 and vB_Eco_mar004NP2.
AB - Bacteriophages infecting Escherichia coli (coliphages) have been used as a proxy for faecal matter and water quality from a variety of environments. However, the diversity of coliphages that is present in seawater remains largely unknown, with previous studies largely focusing on morphological diversity. Here, we isolated and characterized coliphages from three coastal locations in the United Kingdom and Poland. Comparative genomics and phylogenetic analysis of phage isolates facilitated the identification of putative new species within the genera Rb69virus and T5virus and a putative new genus within the subfamily Tunavirinae. Furthermore, genomic and proteomic analysis combined with host range analysis allowed the identification of a putative tail fibre that is likely responsible for the observed differences in host range of phages vB_Eco_mar003J3 and vB_Eco_mar004NP2.
KW - Coliphages/classification
KW - Escherichia coli/genetics
KW - Genome, Viral
KW - Genomics
KW - Host Specificity
KW - Myoviridae/classification
KW - Phylogeny
KW - Poland
KW - Proteomics
KW - Seawater/virology
KW - Siphoviridae/classification
KW - United Kingdom
U2 - 10.1111/1462-2920.14590
DO - 10.1111/1462-2920.14590
M3 - Article
C2 - 30884081
VL - 21
SP - 2112
EP - 2128
JO - Environmental Microbiology
JF - Environmental Microbiology
SN - 1462-2920
IS - 6
ER -