TY - JOUR

T1 - Riding the wave of genomics to investigate aquatic coliphage diversity and activity

AU - Michniewski, Slawomir

AU - Redgwell, Tamsin

AU - Grigonyte, Aurelija

AU - Rihtman, Branko

AU - Aguilo-Ferretjans, Maria

AU - Christie-Oleza, Joseph

AU - Jameson, Eleanor

AU - Scanlan, David J

AU - Millard, Andrew D

N1 - © 2019 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Bacteriophages infecting Escherichia coli (coliphages) have been used as a proxy for faecal matter and water quality from a variety of environments. However, the diversity of coliphages that is present in seawater remains largely unknown, with previous studies largely focusing on morphological diversity. Here, we isolated and characterized coliphages from three coastal locations in the United Kingdom and Poland. Comparative genomics and phylogenetic analysis of phage isolates facilitated the identification of putative new species within the genera Rb69virus and T5virus and a putative new genus within the subfamily Tunavirinae. Furthermore, genomic and proteomic analysis combined with host range analysis allowed the identification of a putative tail fibre that is likely responsible for the observed differences in host range of phages vB_Eco_mar003J3 and vB_Eco_mar004NP2.

AB - Bacteriophages infecting Escherichia coli (coliphages) have been used as a proxy for faecal matter and water quality from a variety of environments. However, the diversity of coliphages that is present in seawater remains largely unknown, with previous studies largely focusing on morphological diversity. Here, we isolated and characterized coliphages from three coastal locations in the United Kingdom and Poland. Comparative genomics and phylogenetic analysis of phage isolates facilitated the identification of putative new species within the genera Rb69virus and T5virus and a putative new genus within the subfamily Tunavirinae. Furthermore, genomic and proteomic analysis combined with host range analysis allowed the identification of a putative tail fibre that is likely responsible for the observed differences in host range of phages vB_Eco_mar003J3 and vB_Eco_mar004NP2.

KW - Coliphages/classification

KW - Escherichia coli/genetics

KW - Genome, Viral

KW - Genomics

KW - Host Specificity

KW - Myoviridae/classification

KW - Phylogeny

KW - Poland

KW - Proteomics

KW - Seawater/virology

KW - Siphoviridae/classification

KW - United Kingdom

U2 - 10.1111/1462-2920.14590

DO - 10.1111/1462-2920.14590

M3 - Article

C2 - 30884081

VL - 21

SP - 2112

EP - 2128

JO - Environmental Microbiology

JF - Environmental Microbiology

SN - 1462-2920

IS - 6

ER -