TY - JOUR

T1 - Structural characterization of lichenysin A components by fast atom bombardment tandem mass spectrometry

AU - Yakimov, M M

AU - Abraham, W R

AU - Meyer, Holger

AU - Laura Giuliano, null

AU - Golyshin, P N

PY - 1999/5/18

Y1 - 1999/5/18

N2 - The structural characterization of the cyclic lipoheptapeptide surfactant lichenysin A components, produced by Bacillus licheniformis strains via the non-ribosomal pathway on a corresponding peptide synthetase, was carried out using a tandem mass spectrometry (MS/MS) under fast atom bombardment (FAB) conditions. Based on the analysis of the collision-induced fragment-ion spectrum of the single charged molecular ions of both native and partially hydrolyzed forms of lipopeptide, a new general structure of lichenysin A components was elucidated. It varies from previously proposed structure by having in the peptide portion of lipopeptide the L-Gln-1 and L-Asp-5 residues instead of L-Glu-1 and L-Asn-5. The verified chemical structure of lichenysin A was found to be reflected in the structural organization of the corresponding lichenysin A synthetase, LchA, described recently.

AB - The structural characterization of the cyclic lipoheptapeptide surfactant lichenysin A components, produced by Bacillus licheniformis strains via the non-ribosomal pathway on a corresponding peptide synthetase, was carried out using a tandem mass spectrometry (MS/MS) under fast atom bombardment (FAB) conditions. Based on the analysis of the collision-induced fragment-ion spectrum of the single charged molecular ions of both native and partially hydrolyzed forms of lipopeptide, a new general structure of lichenysin A components was elucidated. It varies from previously proposed structure by having in the peptide portion of lipopeptide the L-Gln-1 and L-Asp-5 residues instead of L-Glu-1 and L-Asn-5. The verified chemical structure of lichenysin A was found to be reflected in the structural organization of the corresponding lichenysin A synthetase, LchA, described recently.

KW - Bacillus/chemistry

KW - Lipoproteins/chemistry

KW - Molecular Structure

KW - Peptides, Cyclic/chemistry

KW - Spectrometry, Mass, Fast Atom Bombardment

M3 - Article

C2 - 10320810

VL - 1438

SP - 273

EP - 280

JO - Biochimica et Biophysica Acta (BBA)

JF - Biochimica et Biophysica Acta (BBA)

SN - 0006-3002

IS - 2

ER -