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Structural characterization of lichenysin A components by fast atom bombardment tandem mass spectrometry

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Structural characterization of lichenysin A components by fast atom bombardment tandem mass spectrometry. / Yakimov, M M; Abraham, W R; Meyer, Holger et al.
In: Biochimica et Biophysica Acta (BBA), Vol. 1438, No. 2, 18.05.1999, p. 273-80.

Research output: Contribution to journal › Article › peer-review

HarvardHarvard

Yakimov, MM, Abraham, WR, Meyer, H, Laura Giuliano, & Golyshin, PN 1999, 'Structural characterization of lichenysin A components by fast atom bombardment tandem mass spectrometry', Biochimica et Biophysica Acta (BBA), vol. 1438, no. 2, pp. 273-80.

APA

Yakimov, M. M., Abraham, W. R., Meyer, H., Laura Giuliano, & Golyshin, P. N. (1999). Structural characterization of lichenysin A components by fast atom bombardment tandem mass spectrometry. Biochimica et Biophysica Acta (BBA), 1438(2), 273-80.

CBE

Yakimov MM, Abraham WR, Meyer H, Laura Giuliano , Golyshin PN. 1999. Structural characterization of lichenysin A components by fast atom bombardment tandem mass spectrometry. Biochimica et Biophysica Acta (BBA). 1438(2):273-80.

MLA

Yakimov, M M et al. "Structural characterization of lichenysin A components by fast atom bombardment tandem mass spectrometry". Biochimica et Biophysica Acta (BBA). 1999, 1438(2). 273-80.

VancouverVancouver

Yakimov MM, Abraham WR, Meyer H, Laura Giuliano , Golyshin PN. Structural characterization of lichenysin A components by fast atom bombardment tandem mass spectrometry. Biochimica et Biophysica Acta (BBA). 1999 May 18;1438(2):273-80.

Author

Yakimov, M M ; Abraham, W R ; Meyer, Holger et al. / Structural characterization of lichenysin A components by fast atom bombardment tandem mass spectrometry. In: Biochimica et Biophysica Acta (BBA). 1999 ; Vol. 1438, No. 2. pp. 273-80.

RIS

TY - JOUR

T1 - Structural characterization of lichenysin A components by fast atom bombardment tandem mass spectrometry

AU - Yakimov, M M

AU - Abraham, W R

AU - Meyer, Holger

AU - Laura Giuliano, null

AU - Golyshin, P N

PY - 1999/5/18

Y1 - 1999/5/18

N2 - The structural characterization of the cyclic lipoheptapeptide surfactant lichenysin A components, produced by Bacillus licheniformis strains via the non-ribosomal pathway on a corresponding peptide synthetase, was carried out using a tandem mass spectrometry (MS/MS) under fast atom bombardment (FAB) conditions. Based on the analysis of the collision-induced fragment-ion spectrum of the single charged molecular ions of both native and partially hydrolyzed forms of lipopeptide, a new general structure of lichenysin A components was elucidated. It varies from previously proposed structure by having in the peptide portion of lipopeptide the L-Gln-1 and L-Asp-5 residues instead of L-Glu-1 and L-Asn-5. The verified chemical structure of lichenysin A was found to be reflected in the structural organization of the corresponding lichenysin A synthetase, LchA, described recently.

AB - The structural characterization of the cyclic lipoheptapeptide surfactant lichenysin A components, produced by Bacillus licheniformis strains via the non-ribosomal pathway on a corresponding peptide synthetase, was carried out using a tandem mass spectrometry (MS/MS) under fast atom bombardment (FAB) conditions. Based on the analysis of the collision-induced fragment-ion spectrum of the single charged molecular ions of both native and partially hydrolyzed forms of lipopeptide, a new general structure of lichenysin A components was elucidated. It varies from previously proposed structure by having in the peptide portion of lipopeptide the L-Gln-1 and L-Asp-5 residues instead of L-Glu-1 and L-Asn-5. The verified chemical structure of lichenysin A was found to be reflected in the structural organization of the corresponding lichenysin A synthetase, LchA, described recently.

KW - Bacillus/chemistry

KW - Lipoproteins/chemistry

KW - Molecular Structure

KW - Peptides, Cyclic/chemistry

KW - Spectrometry, Mass, Fast Atom Bombardment

M3 - Article

C2 - 10320810

VL - 1438

SP - 273

EP - 280

JO - Biochimica et Biophysica Acta (BBA)

JF - Biochimica et Biophysica Acta (BBA)

SN - 0006-3002

IS - 2

ER -