Population estimates are essential for the conservation of wildlife. However, accurate estimates can be labour-intensive, particularly for small and medium-sized mammals where capture and handling of individuals is often required. Our study aimed to provide a robust and standardised method of determining red squirrel abundance using remotely activated cameras, while also quantifying how red squirrel abundance relates to key habitat characteristics. Fifty sample points were selected in four habitat categories in forests across Anglesey. We found a strong correlation (r = 0.78) between the number of red squirrels live-trapped over ten-days with the number of camera images recorded during a five-day period. Red squirrel abundance was negatively related to stand canopy openness, while the presence of Scots pine and increased tree species diversity were positively related to squirrel abundance. Our results demonstrate that remotely activated cameras can provide a suitable tool for surveying red squirrel abundance and guiding habitat management.