A GAL4-Driver Line Resource for Drosophila Neurobiology

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DOI

  • Arnim Jenett
    Howard Hughes Medical Institute, Ashburn
  • Gerald Rubin
    Howard Hughes Medical Institute, Ashburn
  • Teri T.B. Ngo
    Howard Hughes Medical Institute, Ashburn
  • David Shepherd
  • Christine Murphy
    Howard Hughes Medical Institute, Ashburn
  • Heather Dionne
    Howard Hughes Medical Institute, Ashburn
  • Barrett Pfeiffer
    Howard Hughes Medical Institute, Ashburn
  • Amanda Cavallaro
    Howard Hughes Medical Institute, Ashburn
  • Donald Hall
    Howard Hughes Medical Institute, Ashburn
  • Jennifer Jeter
    Howard Hughes Medical Institute, Ashburn
  • Nirmal Iyer
    Howard Hughes Medical Institute, Ashburn
  • Dona Fetter
    Howard Hughes Medical Institute, Ashburn
  • Joanna Hausenfluck
    Howard Hughes Medical Institute, Ashburn
  • Hanchuan Peng
    Howard Hughes Medical Institute, Ashburn
  • Eric T. Trautman
    Howard Hughes Medical Institute, Ashburn
  • Robert R. Svirskas
    Howard Hughes Medical Institute, Ashburn
  • Eugene W. Meyers
    Howard Hughes Medical Institute, Ashburn
  • Zbigniew R. Iwinski
    Carl Zeiss Microscopy LLC, Thornwood, NY
  • Yoshnori Aso
    Howard Hughes Medical Institute, Ashburn
  • Gina M. DePasquale
    Howard Hughes Medical Institute, Ashburn
  • Adrianne Enos
    Howard Hughes Medical Institute, Ashburn
  • Phuson Hulamm
    Howard Hughes Medical Institute, Ashburn
  • Shing Chun Benny Lam
    Howard Hughes Medical Institute, Ashburn
  • Hsing-Hsi Li
    Howard Hughes Medical Institute, Ashburn
  • Todd Laverty
    Howard Hughes Medical Institute, Ashburn
  • Fuhui Long
    Howard Hughes Medical Institute, Ashburn
  • Lei Qu
    Howard Hughes Medical Institute, Ashburn
  • Sean D. Murphy
    Howard Hughes Medical Institute, Ashburn
  • Conrad Rokicki
    Howard Hughes Medical Institute, Ashburn
  • Todd Safford
    Howard Hughes Medical Institute, Ashburn
  • Kshiti Shaw
    Howard Hughes Medical Institute, Ashburn
  • Julie H. Simpson
    Howard Hughes Medical Institute, Ashburn
  • Allison Sowell
    Howard Hughes Medical Institute, Ashburn
  • Susana Tae
    Howard Hughes Medical Institute, Ashburn
  • Yang Yu
    Howard Hughes Medical Institute, Ashburn
  • Christopher T. Zugates
    Howard Hughes Medical Institute, Ashburn
We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord. We present image data on 6,650 lines. Using both manual and machine-assisted annotation, we describe the expression patterns in the most useful lines. We illustrate the utility of these data for identifying novel neuronal cell types, revealing brain asymmetry, and describing the nature and extent of neuronal shape stereotypy. The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each driving a documented expression pattern, will facilitate the generation of additional constructs for manipulating neuronal function.
Original languageEnglish
Pages (from-to)991-1001
Number of pages10
JournalCell Reports
Volume2
Issue number4
DOIs
Publication statusPublished - 11 Oct 2012
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