Electronic versions

  • M M Yakimov
    Division of Microbiology, GBF National Research Centre for Biotechnology, Braunschweig, Germany.
  • P N Golyshin
  • S Lang
  • E R Moore
  • W R Abraham
  • H Lünsdorf
  • K N Timmis

During screening for biosurfactant-producing, n-alkane-degrading marine bacteria, six heterotrophic bacterial strains were isolated from enriched mixed cultures, obtained from sea water/sediment samples collected near the isle of Borkum (North Sea), using Mihagol-S (C14,15-n-alkanes) as principal carbon source. These Gram-negative, aerobic, rod-shaped bacteria use a limited number of organic compounds, including aliphatic hydrocarbons, volatile fatty acids, and pyruvate and its methyl ether. During cultivation on n-alkanes as sole source of carbon and energy, all strains produced both extracellular and cell-bound surface-active glucose lipids which reduced the surface tension of water from 72 to 29 mN m-1 (16). This novel class of glycolipids was found to be produced only by these strains. The 16S rRNA gene sequence analysis showed that these strains are all members of the gamma-subclass of the Proteobacteria. Their phospholipids ester-linked fatty acid composition was shown to be similar to that of members of the genus Halmonas, although they did not demonstrate a close phylogenetic relationship to any previously described species. On the basis of the information summarized above, a new genus and species, Alcanivorax borkumensis, is described to include these bacteria. Strain SK2T is the type strain of A. borkumensis.

Keywords

  • Base Composition, Base Sequence, DNA, Bacterial, Gram-Negative Bacteria/classification, Molecular Sequence Data, Phenotype, Phylogeny, Seawater/microbiology, Surface-Active Agents/metabolism
Original languageEnglish
Pages (from-to)339-48
Number of pages10
JournalInternational journal of systematic bacteriology
Volume48 Pt 2
DOIs
Publication statusPublished - Apr 1998
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