TY - JOUR

T1 - Bacterial consortium proteomics under 4-chlorosalicylate carbon-limiting conditions

AU - Bobadilla Fazzini, Roberto A

AU - Bielecka, Agata

AU - Quintas, Ana K Poucas

AU - Golyshin, Peter N

AU - Preto, Maria J

AU - Timmis, Kenneth N

AU - dos Santos, Vitor A P Martins

PY - 2009/4

Y1 - 2009/4

N2 - In this study, the stable consortium composed by Pseudomonas reinekei strain MT1 and Achromobacter xylosoxidans strain MT3 (cell numbers in proportion 9:1) was under investigation to reveal bacterial interactions that take place under severe nutrient-limiting conditions. The analysis of steady states in continuous cultures was carried out at the proteome, metabolic profile, and population dynamic levels. Carbon-limiting studies showed a higher metabolic versatility in the community through upregulation of parallel catabolic enzymes (salicylate 5-hydroxylase and 17-fold on 2-keto-4-pentenoate hydratase) indicating a possible alternative carbon routing in the upper degradation pathway highlighting the effect of minor proportions of strain MT3 over the major consortia component strain MT1 with a significant change in the expression levels of the enzymes of the mainly induced biodegradation pathway such as salicylate 1-hydroxylase and catechol 1,2-dioxygenase together with important changes in the outer membrane composition of P. reinekei MT1 under different culture conditions. The study has demonstrated the importance of the outer membrane as a sensing/response protective barrier caused by interspecies interactions highlighting the role of the major outer membrane proteins OprF and porin D in P. reinekei sp. MT1 under the culture conditions tested.

AB - In this study, the stable consortium composed by Pseudomonas reinekei strain MT1 and Achromobacter xylosoxidans strain MT3 (cell numbers in proportion 9:1) was under investigation to reveal bacterial interactions that take place under severe nutrient-limiting conditions. The analysis of steady states in continuous cultures was carried out at the proteome, metabolic profile, and population dynamic levels. Carbon-limiting studies showed a higher metabolic versatility in the community through upregulation of parallel catabolic enzymes (salicylate 5-hydroxylase and 17-fold on 2-keto-4-pentenoate hydratase) indicating a possible alternative carbon routing in the upper degradation pathway highlighting the effect of minor proportions of strain MT3 over the major consortia component strain MT1 with a significant change in the expression levels of the enzymes of the mainly induced biodegradation pathway such as salicylate 1-hydroxylase and catechol 1,2-dioxygenase together with important changes in the outer membrane composition of P. reinekei MT1 under different culture conditions. The study has demonstrated the importance of the outer membrane as a sensing/response protective barrier caused by interspecies interactions highlighting the role of the major outer membrane proteins OprF and porin D in P. reinekei sp. MT1 under the culture conditions tested.

KW - Achromobacter denitrificans/chemistry

KW - Bacterial Outer Membrane Proteins/biosynthesis

KW - Bacterial Proteins/biosynthesis

KW - Catechol 1,2-Dioxygenase/biosynthesis

KW - Hydrolases/biosynthesis

KW - Ketol-Acid Reductoisomerase/biosynthesis

KW - Metabolic Networks and Pathways

KW - Metabolome

KW - Mixed Function Oxygenases/biosynthesis

KW - Oxidative Stress

KW - Peptide Elongation Factors/biosynthesis

KW - Proteome/biosynthesis

KW - Pseudomonas/chemistry

KW - Salicylates/metabolism

KW - Species Specificity

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

U2 - 10.1002/pmic.200800489

DO - 10.1002/pmic.200800489

M3 - Article

C2 - 19382143

VL - 9

SP - 2273

EP - 2285

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 8

ER -