TY - JOUR

T1 - ComA-dependent transcriptional activation of lichenysin A synthetase promoter in Bacillus subtilis cells

AU - Yakimov, M M

AU - Golyshin, P N

PY - 1997/12/31

Y1 - 1997/12/31

N2 - ComA is a DNA-binding activator protein which is required for the transcription of several late-growth phase expressed genes including srfA, an operon needed for the development of genetic competence, efficient sporulation, and surfactin production in Bacillus subtilis (B. subtilis). We show here that the ComA protein can also recognize the promoter regulatory region of the lchA, lichenysin A synthetase operon, found in. Bacillus licheniformis (B. licheniformis) when introduced into B. subtilis cells. Mutational analysis of this region suggests that a palindromic sequence upstream of the lchA promoter may be the target for ComA-dependent transcriptional activation. Considering that the comA operon is present in the B. licheniformis chromosome, we propose the similar mechanism of transcriptional activation of the lichenysin A synthetase operon.

AB - ComA is a DNA-binding activator protein which is required for the transcription of several late-growth phase expressed genes including srfA, an operon needed for the development of genetic competence, efficient sporulation, and surfactin production in Bacillus subtilis (B. subtilis). We show here that the ComA protein can also recognize the promoter regulatory region of the lchA, lichenysin A synthetase operon, found in. Bacillus licheniformis (B. licheniformis) when introduced into B. subtilis cells. Mutational analysis of this region suggests that a palindromic sequence upstream of the lchA promoter may be the target for ComA-dependent transcriptional activation. Considering that the comA operon is present in the B. licheniformis chromosome, we propose the similar mechanism of transcriptional activation of the lichenysin A synthetase operon.

KW - Amino Acid Sequence

KW - Bacillus subtilis/enzymology

KW - Bacterial Proteins/chemistry

KW - DNA, Bacterial/analysis

KW - DNA-Binding Proteins/chemistry

KW - Green Fluorescent Proteins

KW - Ligases/genetics

KW - Luminescent Proteins/genetics

KW - Molecular Sequence Data

KW - Mutagenesis, Site-Directed

KW - Open Reading Frames

KW - Operon

KW - Polymerase Chain Reaction

KW - Promoter Regions, Genetic

KW - Recombinant Fusion Proteins

KW - Transcription, Genetic/drug effects

U2 - 10.1021/bp9700622

DO - 10.1021/bp9700622

M3 - Article

C2 - 9413133

VL - 13

SP - 757

EP - 761

JO - Biotechnology progress

JF - Biotechnology progress

SN - 8756-7938

IS - 6

ER -