TY - CONF

T1 - Coplanar stripline microchamber for electrical detection of live and dead biological cells

AU - Ning, Y.

AU - Multari, C.

AU - Luo, X.

AU - Palego, C.

AU - Molinero, D.

AU - Cheng, X.

AU - Hwang, J.C.

AU - Merla, C.

PY - 2013/10/6

Y1 - 2013/10/6

N2 - An improved microchamber for electrical detection of biological cells was designed, fabricated and tested. The microchamber was formed between a gold coplanar stripline fabricated on a sapphire microscope slide and a single microfluidic channel fabricated in a polydimethylsiloxane cover. Compared with the previous design of a coplanar waveguide on a quartz slide with multiple microfluidic channels, the present microchamber was more sensitive, compact, rugged, and transparent. Tests on Jurkat cells in both time and frequency domains demonstrated single-cell sensing potential, as well as discrimination of live vs. dead cells. The test results could be explained by a simple equivalent-circuit model, which efficiently de-embedded the intrinsic cell properties from solution and electrode effects.

AB - An improved microchamber for electrical detection of biological cells was designed, fabricated and tested. The microchamber was formed between a gold coplanar stripline fabricated on a sapphire microscope slide and a single microfluidic channel fabricated in a polydimethylsiloxane cover. Compared with the previous design of a coplanar waveguide on a quartz slide with multiple microfluidic channels, the present microchamber was more sensitive, compact, rugged, and transparent. Tests on Jurkat cells in both time and frequency domains demonstrated single-cell sensing potential, as well as discrimination of live vs. dead cells. The test results could be explained by a simple equivalent-circuit model, which efficiently de-embedded the intrinsic cell properties from solution and electrode effects.

M3 - Paper

SP - 475

EP - 478

T2 - European Microwave Conference (EuMC), Nurember, October 2013

Y2 - 3 January 0001

ER -