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Double-stranded library preparation for ancient and other degraded samples

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Double-stranded library preparation for ancient and other degraded samples. / Henneberger, Kristin; Barlow, Axel; Paijmans, Johanna.
Ancient DNA: methods and protocols. Second Edition. Humana New York, NY, 2019. p. 65-73.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

HarvardHarvard

Henneberger, K, Barlow, A & Paijmans, J 2019, Double-stranded library preparation for ancient and other degraded samples. in Ancient DNA: methods and protocols. Second Edition. Humana New York, NY, pp. 65-73. https://doi.org/10.1007/978-1-4939-9176-1_8

APA

Henneberger, K., Barlow, A., & Paijmans, J. (2019). Double-stranded library preparation for ancient and other degraded samples. In Ancient DNA: methods and protocols. Second Edition (pp. 65-73). Humana New York, NY. https://doi.org/10.1007/978-1-4939-9176-1_8

CBE

Henneberger K, Barlow A, Paijmans J. 2019. Double-stranded library preparation for ancient and other degraded samples. In Ancient DNA: methods and protocols. Second Edition. Humana New York, NY. pp. 65-73. https://doi.org/10.1007/978-1-4939-9176-1_8

MLA

Henneberger, Kristin, Axel Barlow and Johanna Paijmans "Double-stranded library preparation for ancient and other degraded samples". Ancient DNA: methods and protocols. Second Edition. Humana New York, NY. 2019, 65-73. https://doi.org/10.1007/978-1-4939-9176-1_8

VancouverVancouver

Henneberger K, Barlow A, Paijmans J. Double-stranded library preparation for ancient and other degraded samples. In Ancient DNA: methods and protocols. Second Edition. Humana New York, NY. 2019. p. 65-73 doi: https://doi.org/10.1007/978-1-4939-9176-1_8

Author

Henneberger, Kristin ; Barlow, Axel ; Paijmans, Johanna. / Double-stranded library preparation for ancient and other degraded samples. Ancient DNA: methods and protocols. Second Edition. Humana New York, NY, 2019. pp. 65-73

RIS

TY - CHAP

T1 - Double-stranded library preparation for ancient and other degraded samples

AU - Henneberger, Kristin

AU - Barlow, Axel

AU - Paijmans, Johanna

PY - 2019/3/16

Y1 - 2019/3/16

N2 - High-throughput sequencing (HTS) allows fast and cost-efficient sequencing of ancient DNA (aDNA) without prior information about what sequences should be targeted. One necessary step for HTS is the preparation of a sequencing library. Commercial kits are available for this purpose, but many of these are not suitable for aDNA or other types of damaged DNA. Here, we outline a protocol for HTS library preparation that is optimized for ancient DNA. We report the library conversion rate for a range of input template and adapter concentrations. Our results show that the protocol performs at a high efficiency.

AB - High-throughput sequencing (HTS) allows fast and cost-efficient sequencing of ancient DNA (aDNA) without prior information about what sequences should be targeted. One necessary step for HTS is the preparation of a sequencing library. Commercial kits are available for this purpose, but many of these are not suitable for aDNA or other types of damaged DNA. Here, we outline a protocol for HTS library preparation that is optimized for ancient DNA. We report the library conversion rate for a range of input template and adapter concentrations. Our results show that the protocol performs at a high efficiency.

U2 - https://doi.org/10.1007/978-1-4939-9176-1_8

DO - https://doi.org/10.1007/978-1-4939-9176-1_8

M3 - Chapter

SN - 9781493991754

SP - 65

EP - 73

BT - Ancient DNA: methods and protocols. Second Edition

PB - Humana New York, NY

ER -