Recreational boating is a largely unregulated vector of non-native species and contributes to both initial introduction and secondary spread. As such, marinas and ports often experience high propagule pressure and are hotspots of nonnative species. In many countries, there is little or no legal requirement for marinas to implement biosecurity in day-today operations to reduce the risk of non-native species introduction. Instead, biosecurity is often encouraged and implemented voluntarily meaning uptake may be limited. To understand the range of perceptions of biosecurity within the marina sector, focus groups were conducted as part of a workshop attended by operators from Ireland and Wales. In the first focus group, participants discussed the barriers and drivers to the overall process of designing a biosecurity plan for a marina. A second focus group asked participants to identify strengths and weaknesses of a range of biosecurity tools, such as risk assessments and in-water quarantine berths. Thematic analysis revealed lack of financial resources, in combination with doubt regarding the effectiveness of biosecurity interventions, to be the greatest barriers to uptake. Aligning with good practice and the perceived benefits of a clean environment for business were seen as drivers. Integrating biosecurity into normal marina operations requires bottom up and top down support from customers and relevant authorities respectively. We recommend that alongside testing the effectiveness of biosecurity interventions, researchers should also present robust evidence of cost-effectiveness, and consider and address any potential effects on marina businesses.