Electronic versions

DOI

  • Alexandre B. de Menezes
    University of Liverpool
  • James E McDonald
  • Heather E. Allison
    University of Liverpool
  • Alan J. McCarthy
    University of Liverpool

The relative abundance of micromonosporas in the bacterial communities inhabiting cellulose baits, water columns, and sediments of two freshwater lakes was determined by quantitative PCR (qPCR) of reverse-transcribed 16S rRNA. Micromonospora spp. were shown to be significant members of the active bacterial population colonizing cellulosic substrates in the lake sediment, and their increased prevalence with greater depth was confirmed by enumeration of CFU.

Keywords

  • Bacterial Load, Cellulose, DNA, Bacterial, DNA, Ribosomal, Fresh Water, Geologic Sediments, Micromonospora, Molecular Sequence Data, RNA, Ribosomal, 16S, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Journal Article
Original languageEnglish
Pages (from-to)3495-9
Number of pages5
JournalApplied and Environmental Microbiology
Volume78
Issue number9
DOIs
Publication statusPublished - May 2012
View graph of relations