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Importance of Micromonospora spp. as colonizers of cellulose in freshwater lakes as demonstrated by quantitative reverse transcriptase PCR of 16S rRNA. / de Menezes, Alexandre B.; McDonald, James E; Allison, Heather E. et al.
In: Applied and Environmental Microbiology, Vol. 78, No. 9, 05.2012, p. 3495-9.

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de Menezes AB, McDonald JE, Allison HE, McCarthy AJ. Importance of Micromonospora spp. as colonizers of cellulose in freshwater lakes as demonstrated by quantitative reverse transcriptase PCR of 16S rRNA. Applied and Environmental Microbiology. 2012 May;78(9):3495-9. doi: 10.1128/AEM.07314-11

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de Menezes, Alexandre B. ; McDonald, James E ; Allison, Heather E. et al. / Importance of Micromonospora spp. as colonizers of cellulose in freshwater lakes as demonstrated by quantitative reverse transcriptase PCR of 16S rRNA. In: Applied and Environmental Microbiology. 2012 ; Vol. 78, No. 9. pp. 3495-9.

RIS

TY - JOUR

T1 - Importance of Micromonospora spp. as colonizers of cellulose in freshwater lakes as demonstrated by quantitative reverse transcriptase PCR of 16S rRNA

AU - de Menezes, Alexandre B.

AU - McDonald, James E

AU - Allison, Heather E.

AU - McCarthy, Alan J.

PY - 2012/5

Y1 - 2012/5

N2 - The relative abundance of micromonosporas in the bacterial communities inhabiting cellulose baits, water columns, and sediments of two freshwater lakes was determined by quantitative PCR (qPCR) of reverse-transcribed 16S rRNA. Micromonospora spp. were shown to be significant members of the active bacterial population colonizing cellulosic substrates in the lake sediment, and their increased prevalence with greater depth was confirmed by enumeration of CFU.

AB - The relative abundance of micromonosporas in the bacterial communities inhabiting cellulose baits, water columns, and sediments of two freshwater lakes was determined by quantitative PCR (qPCR) of reverse-transcribed 16S rRNA. Micromonospora spp. were shown to be significant members of the active bacterial population colonizing cellulosic substrates in the lake sediment, and their increased prevalence with greater depth was confirmed by enumeration of CFU.

KW - Bacterial Load

KW - Cellulose

KW - DNA, Bacterial

KW - DNA, Ribosomal

KW - Fresh Water

KW - Geologic Sediments

KW - Micromonospora

KW - Molecular Sequence Data

KW - RNA, Ribosomal, 16S

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Sequence Analysis, DNA

KW - Journal Article

U2 - 10.1128/AEM.07314-11

DO - 10.1128/AEM.07314-11

M3 - Article

C2 - 22389367

VL - 78

SP - 3495

EP - 3499

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 9

ER -