Species’ natural regeneration capacity is an ecological property of plant communities that is key to restoring diversity after disturbances and to conserving the delivery of related ecosystem services within agroecosystems. Reduced diversity of trees and shrubs promoted by conventional and intensive livestock pastureland management can reduce capacity for natural regeneration, negatively affecting current and future ecological processes. We evaluate the relationships between the cover of woody species with different plant traits and the abundance of naturally regenerated seedlings and saplings within conventional pastureland management. Four main dimensions of plant traits (leaf, stem density, canopy height and reproductive variability spectra) were measured for the 76 woody species most commonly found within conventionally managed pastureland in the Mesoamerican region. All these plant traits were correlated with species’ abundance and natural regeneration capacity. Under current practices, there is a risk of decrease in functional diversity of woody components and their capacity to deliver ecosystem services due to loss of species with a low regeneration capacity. The development of livestock management strategies, like agroforestry, natural regeneration management and specifically silvopastoral systems that take into account woody plant traits, are important to conserve current and future agro-biodiversity and potential delivery of ecosystem services in agricultural landscapes.