Inhalation of House Dust and Ozone Alters Systemic Levels of Endothelial Progenitor Cells, Oxidative Stress, and Inflammation in Elderly Subjects
Research output: Contribution to journal › Article › peer-review
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DOI
Ambient air pollution including ozone and especially particulate matter represents important causes of cardiovascular disease. However, there is limited knowledge on indoor air dust with respect to this risk and the potential interactions between dust and ozone. Here, we exposed 23 healthy elderly subjects for 5.5 h, to either clean air, house dust at 275 µg/m3 (diameter < 2.5 µm), ozone at 100 ppb or combined house dust and ozone in a double-blinded randomized cross-over study. The combined house dust and ozone exposure was associated with a 48% (95% CI 24%-65%) decrease as compared with the clean air exposure, in CD34+KDR+ late endothelial progenitor cells (EPCs) per leukocyte in the blood shortly after exposure, whereas none of the single exposures resulted in a significant effect. The combined exposure also increased reactive oxygen species production capacity in granulocytes and monocytes as well as an up-regulation of interleukin-8 mRNA levels in leukocytes. Ozone alone reduced the gene expression of tumor necrosis factor and C-C motif chemokine ligand 2, while dust alone showed no effects. The combined exposure to house dust and ozone also reduced levels of oxidized purines in DNA consistent with concomitant up-regulation of mRNA of the repair enzyme 8-oxoguanine DNA glycosylase. The reduction in late EPCs can be an indicator of cardiovascular risk caused by the combination of pulmonary oxidative stress induced by ozone and the inflammatory potential of the house dust. These data were corroborated with in vitro findings from exposed human macrophages and endothelial cells.
Keywords
- Air Pollution, Indoor/adverse effects, Cross-Over Studies, Double-Blind Method, Dust/analysis, Endothelial Progenitor Cells/cytology, Healthy Volunteers, Humans, Inflammation/blood, Inhalation Exposure/adverse effects, Interleukin-8/blood, Leukocytes/immunology, Oxidative Stress/drug effects, Ozone/toxicity, Particle Size
Original language | English |
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Pages (from-to) | 353-363 |
Number of pages | 11 |
Journal | Toxicological sciences |
Volume | 163 |
Issue number | 2 |
Early online date | 14 May 2018 |
DOIs | |
Publication status | Published - 1 Jun 2018 |
Externally published | Yes |