Research Portal

Klonirovanie gena vnekletochnoĭ RNKazy Bacillus circulans

Research output: Contribution to journal › Article › peer-review

Standard Standard

Klonirovanie gena vnekletochnoĭ RNKazy Bacillus circulans. / Fedorova, N D; Shul'ga, A A; Peredel'chuk, M P et al.
In: Molekuliarnaia biologiia, Vol. 28, No. 2, 01.03.1994, p. 468-71.

Research output: Contribution to journal › Article › peer-review

HarvardHarvard

Fedorova, ND, Shul'ga, AA, Peredel'chuk, MP, Kozharinova, LV, Golyshin, PN, Riabchenko, NF & Kirpichnikov, MP 1994, 'Klonirovanie gena vnekletochnoĭ RNKazy Bacillus circulans', Molekuliarnaia biologiia, vol. 28, no. 2, pp. 468-71.

APA

Fedorova, N. D., Shul'ga, A. A., Peredel'chuk, M. P., Kozharinova, L. V., Golyshin, P. N., Riabchenko, N. F., & Kirpichnikov, M. P. (1994). Klonirovanie gena vnekletochnoĭ RNKazy Bacillus circulans. Molekuliarnaia biologiia, 28(2), 468-71.

CBE

Fedorova ND, Shul'ga AA, Peredel'chuk MP, Kozharinova LV, Golyshin PN, Riabchenko NF, Kirpichnikov MP. 1994. Klonirovanie gena vnekletochnoĭ RNKazy Bacillus circulans. Molekuliarnaia biologiia. 28(2):468-71.

MLA

Fedorova, N D et al. "Klonirovanie gena vnekletochnoĭ RNKazy Bacillus circulans". Molekuliarnaia biologiia. 1994, 28(2). 468-71.

VancouverVancouver

Fedorova ND, Shul'ga AA, Peredel'chuk MP, Kozharinova LV, Golyshin PN, Riabchenko NF et al. Klonirovanie gena vnekletochnoĭ RNKazy Bacillus circulans. Molekuliarnaia biologiia. 1994 Mar 1;28(2):468-71.

Author

Fedorova, N D ; Shul'ga, A A ; Peredel'chuk, M P et al. / Klonirovanie gena vnekletochnoĭ RNKazy Bacillus circulans. In: Molekuliarnaia biologiia. 1994 ; Vol. 28, No. 2. pp. 468-71.

RIS

TY - JOUR

T1 - Klonirovanie gena vnekletochnoĭ RNKazy Bacillus circulans

AU - Fedorova, N D

AU - Shul'ga, A A

AU - Peredel'chuk, M P

AU - Kozharinova, L V

AU - Golyshin, P N

AU - Riabchenko, N F

AU - Kirpichnikov, M P

PY - 1994/3/1

Y1 - 1994/3/1

N2 - The gene for extracellular low molecular weight ribonuclease of Bacillus circulans BCF 247 was cloned. The strain was isolated from permafrost deposits of the Kolyma lowland. The gene for the ribonuclease from Bacillus intermedius (binase) was used as a specific probe. The cloning succeeded only in the E. coli strain producing the inhibitor of ribonuclease form Bacillus amyloliquefaciens. Selected clones secreted the active ribonuclease into the growth media. Deletion derivatives of the parental recombinant plasmid were constructed. The smallest DNA fragment which enclosed a functional ribonuclease gene in E. coli was determined to be 0.6 kb in length.

AB - The gene for extracellular low molecular weight ribonuclease of Bacillus circulans BCF 247 was cloned. The strain was isolated from permafrost deposits of the Kolyma lowland. The gene for the ribonuclease from Bacillus intermedius (binase) was used as a specific probe. The cloning succeeded only in the E. coli strain producing the inhibitor of ribonuclease form Bacillus amyloliquefaciens. Selected clones secreted the active ribonuclease into the growth media. Deletion derivatives of the parental recombinant plasmid were constructed. The smallest DNA fragment which enclosed a functional ribonuclease gene in E. coli was determined to be 0.6 kb in length.

KW - Bacillus/enzymology

KW - Base Sequence

KW - Cloning, Molecular

KW - DNA Primers

KW - Escherichia coli/genetics

KW - Molecular Sequence Data

KW - Plasmids

KW - Ribonucleases/genetics

M3 - Erthygl

C2 - 8183279

VL - 28

SP - 468

EP - 471

JO - Molekuliarnaia biologiia

JF - Molekuliarnaia biologiia

SN - 0026-8984

IS - 2

ER -