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Microscale Temperature Measurements Within Specific Exposure Systems for Real-Time Cellular Characterization

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  • Amani Nefzi
    University of Limoges
  • Rosa Orlacchio
    University of Limoges
  • Lynn Carr
    School of Computer Science & Engineering
  • Noelle Lewis
    Université Bordeaux
  • Yann Percherancier
    Université Bordeaux
  • Philippe Leveque
    University of Limoges
  • Delia Arnaud-Cormos
    Institut Universitaire de France (IUF), Paris
The precise characterization of electromagnetic fields exposure systems requires accurate dosimetry from macroscale down to the cellular level. In this study, two different exposure devices based on microelectrodes arrays for real-time cellular characterization, namely a Micro-Electrode Array (MEA) and an impedancemetry well with interdigitated electrodes were considered. For the first time, the heating induced by the exposure to radiofrequency in the electrodes vicinity was retrieved from the fluorescence intensity of Rhodamine B (Rhod-B), which linearly changes as a function of the temperature variation. Our results confirm that the use of Rhod-B represents a reliable technique to obtain temperature information at the microscopic scale.
Original languageEnglish
Journal2020 IEEE MTT-S International Microwave Biomedical Conference (IMBioC)
DOIs
Publication statusPublished - 14 Dec 2020
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