Microscale Temperature Measurements Within Specific Exposure Systems for Real-Time Cellular Characterization
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In: 2020 IEEE MTT-S International Microwave Biomedical Conference (IMBioC), 14.12.2020.
Research output: Contribution to journal › Article
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TY - JOUR
T1 - Microscale Temperature Measurements Within Specific Exposure Systems for Real-Time Cellular Characterization
AU - Nefzi, Amani
AU - Orlacchio, Rosa
AU - Carr, Lynn
AU - Lewis, Noelle
AU - Percherancier, Yann
AU - Leveque, Philippe
AU - Arnaud-Cormos, Delia
PY - 2020/12/14
Y1 - 2020/12/14
N2 - The precise characterization of electromagnetic fields exposure systems requires accurate dosimetry from macroscale down to the cellular level. In this study, two different exposure devices based on microelectrodes arrays for real-time cellular characterization, namely a Micro-Electrode Array (MEA) and an impedancemetry well with interdigitated electrodes were considered. For the first time, the heating induced by the exposure to radiofrequency in the electrodes vicinity was retrieved from the fluorescence intensity of Rhodamine B (Rhod-B), which linearly changes as a function of the temperature variation. Our results confirm that the use of Rhod-B represents a reliable technique to obtain temperature information at the microscopic scale.
AB - The precise characterization of electromagnetic fields exposure systems requires accurate dosimetry from macroscale down to the cellular level. In this study, two different exposure devices based on microelectrodes arrays for real-time cellular characterization, namely a Micro-Electrode Array (MEA) and an impedancemetry well with interdigitated electrodes were considered. For the first time, the heating induced by the exposure to radiofrequency in the electrodes vicinity was retrieved from the fluorescence intensity of Rhodamine B (Rhod-B), which linearly changes as a function of the temperature variation. Our results confirm that the use of Rhod-B represents a reliable technique to obtain temperature information at the microscopic scale.
U2 - 10.1109/imbioc47321.2020.9384900
DO - 10.1109/imbioc47321.2020.9384900
M3 - Article
JO - 2020 IEEE MTT-S International Microwave Biomedical Conference (IMBioC)
JF - 2020 IEEE MTT-S International Microwave Biomedical Conference (IMBioC)
ER -