TY - JOUR

T1 - Molecular methods to study complex microbial communities

AU - Vieites, José M

AU - Guazzaroni, María-Eugenia

AU - Beloqui, Ana

AU - Golyshin, Peter N

AU - Ferrer, Manuel

PY - 2010

Y1 - 2010

N2 - Microbes, which constitute a major fraction of the total biomass, are the main source of biodiversity on our Planet and play an essential role in maintaining global processes, which ultimately regulate the functioning of the Biosphere. Recent emergence of "metagenomics" allows for the analysis of microbial communities without tedious cultivation efforts. Metagenomics approach is analogous to the genomics with the difference that it does not deal with the single genome from a clone or microbe cultured or characterized in laboratory, but rather with that from the entire microbial community present in an environmental sample; it is the community genome. Global understanding by metagenomics depends essentially on the possibility of isolating the entire bulk DNA and identifying the genomes, genes, and proteins more relevant to each of the environmental sample under investigation. Following on this, in this chapter, we provide an analysis of methods available to isolate environmental DNA and to establish metagenomic libraries that can further be used for extensive activity screens.

AB - Microbes, which constitute a major fraction of the total biomass, are the main source of biodiversity on our Planet and play an essential role in maintaining global processes, which ultimately regulate the functioning of the Biosphere. Recent emergence of "metagenomics" allows for the analysis of microbial communities without tedious cultivation efforts. Metagenomics approach is analogous to the genomics with the difference that it does not deal with the single genome from a clone or microbe cultured or characterized in laboratory, but rather with that from the entire microbial community present in an environmental sample; it is the community genome. Global understanding by metagenomics depends essentially on the possibility of isolating the entire bulk DNA and identifying the genomes, genes, and proteins more relevant to each of the environmental sample under investigation. Following on this, in this chapter, we provide an analysis of methods available to isolate environmental DNA and to establish metagenomic libraries that can further be used for extensive activity screens.

KW - Bacteria/genetics

KW - Biodiversity

KW - Cosmids/genetics

KW - DNA/analysis

KW - Environment

KW - Environmental Microbiology

KW - Gene Library

KW - Metagenome/genetics

KW - Metagenomics/methods

KW - RNA, Ribosomal, 16S/analysis

U2 - 10.1007/978-1-60761-823-2_1

DO - 10.1007/978-1-60761-823-2_1

M3 - Article

C2 - 20830553

VL - 668

SP - 1

EP - 37

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -