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  • post print Purification and polymerisation of microbial d-lactic acid from DDGS hydrolysates fermentation

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    Embargo ends: 15/06/21

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A multi-step process was developed for microbial d-lactic acid purification, followed by poly-d-lactic acid (PDLA) synthesis via azeotropic polycondensation process. Several anion exchange resins were screened for their binding capacity using model lactic acid solutions. Amberlite® IRA67 (weak base anion exchange resin) showed the highest lactic acid adsorption, with maximum adsorption capacity, qmax, of 136.11 mg lactic acid / g of resin, and was further selected to purify D-lactic acid from DDGS hydrolysates through a three-step process; (1) treatment with 7% w/v activated carbon, (2) acidification of fermentation broth (Amberlite® IRA120) and (3) adsorption of lactic acid by anion exchange (Amberlite® IRA67). At the end of the purification process, 80.4% (w/w) D-lactic acid was recovered with 91.8% (w/w) purity, indicating the effectiveness of the developed downstream process. Furthermore, a clear yellowish solid polymer with a molecular weight of 3010 Da was obtained, suitable for applications in biomedical and agricultural sectors.
Original languageEnglish
Article number107265
JournalBiochemical Engineering Journal
Volume150
Early online date15 Jun 2019
DOIs
Publication statusPublished - 15 Oct 2019
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