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Purification and polymerisation of microbial D-lactic acid from DDGS hydrolysates fermentation

Research output: Contribution to journal › Article › peer-review

Standard Standard

Purification and polymerisation of microbial D-lactic acid from DDGS hydrolysates fermentation. / Zaini, Nurul Aqilah Mohd; Chatzifragkou, Afroditi; Tverezovskiy, Viacheslav et al.
In: Biochemical Engineering Journal, Vol. 150, 107265, 15.10.2019.

Research output: Contribution to journal › Article › peer-review

HarvardHarvard

Zaini, NAM, Chatzifragkou, A, Tverezovskiy, V & Charalampopoulos, D 2019, 'Purification and polymerisation of microbial D-lactic acid from DDGS hydrolysates fermentation', Biochemical Engineering Journal, vol. 150, 107265. https://doi.org/10.1016/j.bej.2019.107265

APA

Zaini, N. A. M., Chatzifragkou, A., Tverezovskiy, V., & Charalampopoulos, D. (2019). Purification and polymerisation of microbial D-lactic acid from DDGS hydrolysates fermentation. Biochemical Engineering Journal, 150, Article 107265. https://doi.org/10.1016/j.bej.2019.107265

CBE

Zaini NAM, Chatzifragkou A, Tverezovskiy V, Charalampopoulos D. 2019. Purification and polymerisation of microbial D-lactic acid from DDGS hydrolysates fermentation. Biochemical Engineering Journal. 150:Article 107265. https://doi.org/10.1016/j.bej.2019.107265

MLA

Zaini, Nurul Aqilah Mohd et al. "Purification and polymerisation of microbial D-lactic acid from DDGS hydrolysates fermentation". Biochemical Engineering Journal. 2019. 150. https://doi.org/10.1016/j.bej.2019.107265

VancouverVancouver

Zaini NAM, Chatzifragkou A, Tverezovskiy V, Charalampopoulos D. Purification and polymerisation of microbial D-lactic acid from DDGS hydrolysates fermentation. Biochemical Engineering Journal. 2019 Oct 15;150:107265. Epub 2019 Jun 15. doi: 10.1016/j.bej.2019.107265

Author

Zaini, Nurul Aqilah Mohd ; Chatzifragkou, Afroditi ; Tverezovskiy, Viacheslav et al. / Purification and polymerisation of microbial D-lactic acid from DDGS hydrolysates fermentation. In: Biochemical Engineering Journal. 2019 ; Vol. 150.

RIS

TY - JOUR

T1 - Purification and polymerisation of microbial D-lactic acid from DDGS hydrolysates fermentation

AU - Zaini, Nurul Aqilah Mohd

AU - Chatzifragkou, Afroditi

AU - Tverezovskiy, Viacheslav

AU - Charalampopoulos, Dimitris

PY - 2019/10/15

Y1 - 2019/10/15

N2 - A multi-step process was developed for microbial d-lactic acid purification, followed by poly-d-lactic acid (PDLA) synthesis via azeotropic polycondensation process. Several anion exchange resins were screened for their binding capacity using model lactic acid solutions. Amberlite® IRA67 (weak base anion exchange resin) showed the highest lactic acid adsorption, with maximum adsorption capacity, qmax, of 136.11 mg lactic acid / g of resin, and was further selected to purify D-lactic acid from DDGS hydrolysates through a three-step process; (1) treatment with 7% w/v activated carbon, (2) acidification of fermentation broth (Amberlite® IRA120) and (3) adsorption of lactic acid by anion exchange (Amberlite® IRA67). At the end of the purification process, 80.4% (w/w) D-lactic acid was recovered with 91.8% (w/w) purity, indicating the effectiveness of the developed downstream process. Furthermore, a clear yellowish solid polymer with a molecular weight of 3010 Da was obtained, suitable for applications in biomedical and agricultural sectors.

AB - A multi-step process was developed for microbial d-lactic acid purification, followed by poly-d-lactic acid (PDLA) synthesis via azeotropic polycondensation process. Several anion exchange resins were screened for their binding capacity using model lactic acid solutions. Amberlite® IRA67 (weak base anion exchange resin) showed the highest lactic acid adsorption, with maximum adsorption capacity, qmax, of 136.11 mg lactic acid / g of resin, and was further selected to purify D-lactic acid from DDGS hydrolysates through a three-step process; (1) treatment with 7% w/v activated carbon, (2) acidification of fermentation broth (Amberlite® IRA120) and (3) adsorption of lactic acid by anion exchange (Amberlite® IRA67). At the end of the purification process, 80.4% (w/w) D-lactic acid was recovered with 91.8% (w/w) purity, indicating the effectiveness of the developed downstream process. Furthermore, a clear yellowish solid polymer with a molecular weight of 3010 Da was obtained, suitable for applications in biomedical and agricultural sectors.

U2 - 10.1016/j.bej.2019.107265

DO - 10.1016/j.bej.2019.107265

M3 - Article

VL - 150

JO - Biochemical Engineering Journal

JF - Biochemical Engineering Journal

SN - 1369-703X

M1 - 107265

ER -