The activity of the gluconate-H+ symporter of Schizosaccharomyces pombe cells is down-regulated by D-glucose and exogenous cAMP
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DOI
Schizosaccharomyces pombe cells take up D-gluconate, as an alternative carbon source for growth, during glucose starvation or when cultured on glycerol-containing medium. Gluconate uptake is not detectable while cells are growing logarithmically on glucose. The addition of D-glucose as well as its non-metabolizable analogues to glycerol-grown cells causes an immediate loss of gluconate transport within 1 min. The reversible down-regulation of the gluconate carrier occurs after glucose has been internalized. This regulation is triggered not only by D-glucose but also by extracellular cAMP even in the absence of the cAMP-dependent protein kinase (PKA1).
Keywords
- Biological Transport, Carrier Proteins, Cyclic AMP, Cyclic AMP-Dependent Protein Kinases, Gluconates, Glucose, Kinetics, Schizosaccharomyces, Comparative Study, Journal Article
| Original language | English |
|---|---|
| Pages (from-to) | 272-6 |
| Number of pages | 5 |
| Journal | Febs Letters |
| Volume | 395 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - 21 Oct 1996 |