TY - JOUR

T1 - The activity of the gluconate-H+ symporter of Schizosaccharomyces pombe cells is down-regulated by D-glucose and exogenous cAMP

AU - Caspari, Thomas

AU - Urlinger, Stefanie

PY - 1996/10/21

Y1 - 1996/10/21

N2 - Schizosaccharomyces pombe cells take up D-gluconate, as an alternative carbon source for growth, during glucose starvation or when cultured on glycerol-containing medium. Gluconate uptake is not detectable while cells are growing logarithmically on glucose. The addition of D-glucose as well as its non-metabolizable analogues to glycerol-grown cells causes an immediate loss of gluconate transport within 1 min. The reversible down-regulation of the gluconate carrier occurs after glucose has been internalized. This regulation is triggered not only by D-glucose but also by extracellular cAMP even in the absence of the cAMP-dependent protein kinase (PKA1).

AB - Schizosaccharomyces pombe cells take up D-gluconate, as an alternative carbon source for growth, during glucose starvation or when cultured on glycerol-containing medium. Gluconate uptake is not detectable while cells are growing logarithmically on glucose. The addition of D-glucose as well as its non-metabolizable analogues to glycerol-grown cells causes an immediate loss of gluconate transport within 1 min. The reversible down-regulation of the gluconate carrier occurs after glucose has been internalized. This regulation is triggered not only by D-glucose but also by extracellular cAMP even in the absence of the cAMP-dependent protein kinase (PKA1).

KW - Biological Transport

KW - Carrier Proteins

KW - Cyclic AMP

KW - Cyclic AMP-Dependent Protein Kinases

KW - Gluconates

KW - Glucose

KW - Kinetics

KW - Schizosaccharomyces

KW - Comparative Study

KW - Journal Article

U2 - 10.1016/0014-5793(96)01052-6

DO - 10.1016/0014-5793(96)01052-6

M3 - Article

C2 - 8898110

VL - 395

SP - 272

EP - 276

JO - Febs Letters

JF - Febs Letters

SN - 0014-5793

IS - 2-3

ER -