A high-density genetic map and molecular sex-typing assay for gerbils

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A high-density genetic map and molecular sex-typing assay for gerbils. / Brekke, Thomas; Mulley, John; Steele, Katherine; Denver, Megan; Thom, Angharad; Supriya, Sushmita.

In: Mammalian Genome, Vol. 30, No. 3-4, 04.2019, p. 63-70.

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Brekke, Thomas ; Mulley, John ; Steele, Katherine ; Denver, Megan ; Thom, Angharad ; Supriya, Sushmita. / A high-density genetic map and molecular sex-typing assay for gerbils. In: Mammalian Genome. 2019 ; Vol. 30, No. 3-4. pp. 63-70.

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TY - JOUR

T1 - A high-density genetic map and molecular sex-typing assay for gerbils

AU - Brekke, Thomas

AU - Mulley, John

AU - Steele, Katherine

AU - Denver, Megan

AU - Thom, Angharad

AU - Supriya, Sushmita

PY - 2019/4

Y1 - 2019/4

N2 - We constructed a high-density genetic map for Mongolian gerbils (Meriones unguiculatus). We genotyped 137 F2 individuals with a genotype-by-sequencing (GBS) approach at over 10,000 loci and built the genetic map using a two-step approach. First, we chose the highest-quality set of 485 markers to construct a robust map of 1239 cM with 22 linkage groups as expected from the published karyotype. Second, we added an additional 5449 markers onto the map based on their genotype similarity with the original markers. We used the final marker set to assemble 1140 genomic scaffolds (containing ~ 20% of annotated genes) into a chromosome-level assembly. We used both genetic linkage and relative sequencing coverage in males and females to identify X- and Y-chromosome scaffolds and from these we designed a robust and internally-controlled PCR assay to determine sex. This assay will facilitate early stage sex-typing of embryonic and young gerbils which is difficult using current visual methods

AB - We constructed a high-density genetic map for Mongolian gerbils (Meriones unguiculatus). We genotyped 137 F2 individuals with a genotype-by-sequencing (GBS) approach at over 10,000 loci and built the genetic map using a two-step approach. First, we chose the highest-quality set of 485 markers to construct a robust map of 1239 cM with 22 linkage groups as expected from the published karyotype. Second, we added an additional 5449 markers onto the map based on their genotype similarity with the original markers. We used the final marker set to assemble 1140 genomic scaffolds (containing ~ 20% of annotated genes) into a chromosome-level assembly. We used both genetic linkage and relative sequencing coverage in males and females to identify X- and Y-chromosome scaffolds and from these we designed a robust and internally-controlled PCR assay to determine sex. This assay will facilitate early stage sex-typing of embryonic and young gerbils which is difficult using current visual methods

U2 - 10.1007/s00335-019-09799-z

DO - 10.1007/s00335-019-09799-z

M3 - Article

VL - 30

SP - 63

EP - 70

JO - Mammalian Genome

T2 - Mammalian Genome

JF - Mammalian Genome

SN - 0938-8990

IS - 3-4

ER -