Buwchitin: A Ruminal Peptide with Antimicrobial Potential against Enterococcus faecalis

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  • Linda B. Oyama
    Aberystwyth University
  • Jean-Andrien Crochet
    Aberystwyth University
  • Joan E. Edwards
    Aberystwyth University
  • Susan E. Girdwood
    Aberystwyth University
  • Alan R. Cookson
    Aberystwyth University
  • Narcis Fernandez-Fuentes
    Aberystwyth University
  • Kai Hilpert
    St George's University of London
  • Peter Golyshin
  • Olga Golyshina
  • Florence Prive
  • Matthiass Hess
    University of California, Davis
  • Hilario C. Mantovani
    Universidade Federal de Viçosa
  • Christopher J. Creevey
    Aberystwyth University
  • Sharon A. Huws
    Queen's University, Belfast
Antimicrobial peptides (AMPs) are gaining popularity as alternatives for treatment of bacterial infections and recent advances in omics technologies provide new platforms for AMP discovery. We sought to determine the antibacterial activity of a novel antimicrobial peptide, buwchitin, against Enterococcus faecalis. Buwchitin was identified from a rumen bacterial metagenome library, cloned, expressed and purified. The antimicrobial activity of the recombinant peptide was assessed using a broth microdilution susceptibility assay to determine the peptide's killing kinetics against selected bacterial strains. The killing mechanism of buwchitin was investigated further by monitoring its ability to cause membrane depolarization (diSC3(5) method) and morphological changes in E. faecalis cells. Transmission electron micrographs of buwchitin treated E. faecalis cells showed intact outer membranes with blebbing, but no major damaging effects and cell morphology changes. Buwchitin had negligible cytotoxicity against defibrinated sheep erythrocytes. Although no significant membrane leakage and depolarization was observed, buwchitin at minimum inhibitory concentration (MIC) was bacteriostatic against E. faecalis cells and inhibited growth in vitro by 70% when compared to untreated cells. These findings suggest that buwchitin, a rumen derived peptide, has potential for antimicrobial activity against E. faecalis.
Original languageEnglish
JournalFrontiers in Chemistry
Publication statusPublished - 12 Jul 2017

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