Effect of novel biosurfactants on biodegradation of polychlorinated biphenyls by pure and mixed bacterial cultures
Research output: Contribution to journal › Article › peer-review
Standard Standard
In: New microbiologica, Vol. 22, No. 3, 07.1999, p. 257-67.
Research output: Contribution to journal › Article › peer-review
HarvardHarvard
APA
CBE
MLA
VancouverVancouver
Author
RIS
TY - JOUR
T1 - Effect of novel biosurfactants on biodegradation of polychlorinated biphenyls by pure and mixed bacterial cultures
AU - Golyshin, P M
AU - Fredrickson, H L
AU - Giuliano, L
AU - Rothmel, R
AU - Timmis, K N
AU - Yakimov, M M
PY - 1999/7
Y1 - 1999/7
N2 - A study was conducted to determine the potential positive effect of novel biosurfactants on the enhancement of Aroclor 1248 metabolization in both in vitro and in situ experiments. Among two lipopeptides tested the highest activity was found in experiments with a hydrolytically opened form of lichenysin A. Lichenysin A itself did not enhance the degradation activity of chosen microorganism-degraders and in most cases inhibited their PCB mineralization rates. Glucolipid surfactant from marine bacterium Alcanivorax borkumensis showed in several tests a strong enhancing effect on microbial metabolization of Aroclor 1248 congeners. Biosurfactants appeared to act very specifically, i.e. depending on strain and concentration used. Experiments set up with soil samples did not give a clear answer whether bioemulsifiers applied at low concentration could sufficiently increase the rates of biodegradation in situ. Only A. borkumiensis glucose lipid caused the most marked enhancement of Aroclor 1248 metabolization in soil microcosm. We suggest that taking into account the specificity of surface- and biological activities of various biosurfactants they may promote the mineralization of sorbed PCBs in polluted soils, when the optimized biosurfactant-degrader combination is used.
AB - A study was conducted to determine the potential positive effect of novel biosurfactants on the enhancement of Aroclor 1248 metabolization in both in vitro and in situ experiments. Among two lipopeptides tested the highest activity was found in experiments with a hydrolytically opened form of lichenysin A. Lichenysin A itself did not enhance the degradation activity of chosen microorganism-degraders and in most cases inhibited their PCB mineralization rates. Glucolipid surfactant from marine bacterium Alcanivorax borkumensis showed in several tests a strong enhancing effect on microbial metabolization of Aroclor 1248 congeners. Biosurfactants appeared to act very specifically, i.e. depending on strain and concentration used. Experiments set up with soil samples did not give a clear answer whether bioemulsifiers applied at low concentration could sufficiently increase the rates of biodegradation in situ. Only A. borkumiensis glucose lipid caused the most marked enhancement of Aroclor 1248 metabolization in soil microcosm. We suggest that taking into account the specificity of surface- and biological activities of various biosurfactants they may promote the mineralization of sorbed PCBs in polluted soils, when the optimized biosurfactant-degrader combination is used.
KW - Aroclors/metabolism
KW - Bacteria/metabolism
KW - Bacterial Proteins/pharmacology
KW - Biodegradation, Environmental/drug effects
KW - Glycolipids/pharmacology
KW - Lipopeptides
KW - Lipoproteins/pharmacology
KW - Peptides, Cyclic/pharmacology
KW - Soil Microbiology
KW - Soil Pollutants/metabolism
KW - Surface-Active Agents/pharmacology
M3 - Article
C2 - 10423745
VL - 22
SP - 257
EP - 267
JO - New microbiologica
JF - New microbiologica
SN - 1121-7138
IS - 3
ER -