Rapid and accurate estimation of forest biomass is essential to drive sustainable management of forests. Field-based measurements of forest above-ground biomass (AGB) can be costly and difficult to conduct. Multi-source remote sensing data offers potential to improve the accuracy of modelled AGB predictions. Here, four machine learning methods: Random Forest (RF), Gradient Boosting Decision Tree (GBDT), Classification and Regression Trees (CART) and Minimum Distance (MD) were used to construct forest AGB models of Taiyue Mountain forest, Shanxi Province, China using single and multi-sourced remote sensing data and the Google Earth Engine platform. Results showed that the machine learning method that most accurately predicted AGB was GBDT and spectral index for coniferous (R2=0.99; RMSE=65.52 Mg/ha), broadleaved (R2=0.97; RMSE=29.14 Mg/ha), and mixed species (R2=0.97; RMSE=81.12 Mg/ha) forest types. Models constructed using bivariate variable combinations that included the spectral index improved the AGB estimation accuracy of mixed species (R2=0.99; RMSE=59.52 Mg/ha) forest types and reduced slightly the accuracy of coniferous (R2=0.99; RMSE=101.46 Mg/ha), and broadleaved (R2=0.97; RMSE=37.59 Mg/ha) forest AGB estimation. Overall, parameterising machine learning algorithms with multi-source remote sensing variables can improve the prediction accuracy of mixed species forests.