TY - JOUR

T1 - The genetics of the repaor of 5-azacytidine-mediated DNA damage in the fission yeast Schizosaccharomyces pombe.

AU - Hegde, V

AU - Mcfarlane, Ramsay

AU - Taylor, E.M.

AU - Price, C

PY - 1996/6/1

Y1 - 1996/6/1

N2 - We have recently demonstrated that Schizosaccharomyces pombe cells treated with the nucleoside analogue 5-azacytidine (5-azaC) require previously characterised G2 checkpoint mechanisms for survival. Here we present a survey of known DNA repair mutations which defines those genes required for survival in the presence of 5-azaC. Using a combination of single-mutant and epistasis analyses we find that the excision, mismatch and recombinational repair pathways are all required in some degree for the repair of 5-azaC-mediated DNA damage. There are distinct differences in the epistatic interactions of several of the repair mutations with respect to 5-azaC-mediated DNA damage relative to UV-mediated DNA damage.

AB - We have recently demonstrated that Schizosaccharomyces pombe cells treated with the nucleoside analogue 5-azacytidine (5-azaC) require previously characterised G2 checkpoint mechanisms for survival. Here we present a survey of known DNA repair mutations which defines those genes required for survival in the presence of 5-azaC. Using a combination of single-mutant and epistasis analyses we find that the excision, mismatch and recombinational repair pathways are all required in some degree for the repair of 5-azaC-mediated DNA damage. There are distinct differences in the epistatic interactions of several of the repair mutations with respect to 5-azaC-mediated DNA damage relative to UV-mediated DNA damage.

U2 - 10.1007/BF02172377

DO - 10.1007/BF02172377

M3 - Article

VL - 251

SP - 483

EP - 492

JO - Molecular Genetics and Genomics

JF - Molecular Genetics and Genomics

SN - 1617-4623

IS - 4

ER -