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The rumen microbiome: an underexplored resource for novel antimicrobial discovery

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The rumen microbiome: an underexplored resource for novel antimicrobial discovery. / Oyama, Linda B.; Girdwood, Susan E.; Cookson, Alan R. et al.
In: NPJ Biofilms and Microbiomes, Vol. 3, 33 , 01.12.2017.

Research output: Contribution to journal › Article › peer-review

HarvardHarvard

Oyama, LB, Girdwood, SE, Cookson, AR, Fernanadez-Fuentes, N, Prive, F, Vallin, HE, Wilkinson, TJ, Golyshin, P, Golyshina, O, Mikut, R, Hilpert, K, Richards, J, Wootton, M, Edwards, JE, Maresca, M, Perrier, J, Lundy, FT, Luo, Y, Zhou, M, Hess, M, Mantovani, HC, Creevey, CJ & Huws, SA 2017, 'The rumen microbiome: an underexplored resource for novel antimicrobial discovery', NPJ Biofilms and Microbiomes, vol. 3, 33 . https://doi.org/10.1038/s41522-017-0042-1

APA

Oyama, L. B., Girdwood, S. E., Cookson, A. R., Fernanadez-Fuentes, N., Prive, F., Vallin, H. E., Wilkinson, T. J., Golyshin, P., Golyshina, O., Mikut, R., Hilpert, K., Richards, J., Wootton, M., Edwards, J. E., Maresca, M., Perrier, J., Lundy, F. T., Luo, Y., Zhou, M., ... Huws, S. A. (2017). The rumen microbiome: an underexplored resource for novel antimicrobial discovery. NPJ Biofilms and Microbiomes, 3, Article 33 . https://doi.org/10.1038/s41522-017-0042-1

CBE

Oyama LB, Girdwood SE, Cookson AR, Fernanadez-Fuentes N, Prive F, Vallin HE, Wilkinson TJ, Golyshin P, Golyshina O, Mikut R, et al. 2017. The rumen microbiome: an underexplored resource for novel antimicrobial discovery. NPJ Biofilms and Microbiomes. 3:Article 33 . https://doi.org/10.1038/s41522-017-0042-1

MLA

Oyama, Linda B. et al. "The rumen microbiome: an underexplored resource for novel antimicrobial discovery". NPJ Biofilms and Microbiomes. 2017. 3. https://doi.org/10.1038/s41522-017-0042-1

VancouverVancouver

Oyama LB, Girdwood SE, Cookson AR, Fernanadez-Fuentes N, Prive F, Vallin HE et al. The rumen microbiome: an underexplored resource for novel antimicrobial discovery. NPJ Biofilms and Microbiomes. 2017 Dec 1;3:33 . doi: 10.1038/s41522-017-0042-1

Author

Oyama, Linda B. ; Girdwood, Susan E. ; Cookson, Alan R. et al. / The rumen microbiome : an underexplored resource for novel antimicrobial discovery. In: NPJ Biofilms and Microbiomes. 2017 ; Vol. 3.

RIS

TY - JOUR

T1 - The rumen microbiome

T2 - an underexplored resource for novel antimicrobial discovery

AU - Oyama, Linda B.

AU - Girdwood, Susan E.

AU - Cookson, Alan R.

AU - Fernanadez-Fuentes, Narcis

AU - Prive, Florence

AU - Vallin, Hannah E.

AU - Wilkinson, Toby J.

AU - Golyshin, Peter

AU - Golyshina, Olga

AU - Mikut, Ralf

AU - Hilpert, Kai

AU - Richards, Jennifer

AU - Wootton, Mandy

AU - Edwards, Joan E.

AU - Maresca, Marc

AU - Perrier, Josette

AU - Lundy, Fionnuala T.

AU - Luo, Yu

AU - Zhou, Mei

AU - Hess, Matthias

AU - Mantovani, Hilario C.

AU - Creevey, Christopher J.

AU - Huws, Sharon A.

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Antimicrobial peptides (AMPs) are promising drug candidates to target multi-drug resistant bacteria. The rumen microbiome presents an underexplored resource for the discovery of novel microbial enzymes and metabolites, including AMPs. Using functional screening and computational approaches, we identified 181 potentially novel AMPs from a rumen bacterial metagenome. Here, we show that three of the selected AMPs (Lynronne-1, Lynronne-2 and Lynronne-3) were effective against numerous bacterial pathogens, including methicillin-resistant Staphylococcus aureus (MRSA). No decrease in MRSA susceptibility was observed after 25 days of sub-lethal exposure to these AMPs. The AMPs bound preferentially to bacterial membrane lipids and induced membrane permeability leading to cytoplasmic leakage. Topical administration of Lynronne-1 (10% w/v) to a mouse model of MRSA wound infection elicited a significant reduction in bacterial counts, which was comparable to treatment with 2% mupirocin ointment. Our findings indicate that the rumen microbiome may provide viable alternative antimicrobials for future therapeutic application.

AB - Antimicrobial peptides (AMPs) are promising drug candidates to target multi-drug resistant bacteria. The rumen microbiome presents an underexplored resource for the discovery of novel microbial enzymes and metabolites, including AMPs. Using functional screening and computational approaches, we identified 181 potentially novel AMPs from a rumen bacterial metagenome. Here, we show that three of the selected AMPs (Lynronne-1, Lynronne-2 and Lynronne-3) were effective against numerous bacterial pathogens, including methicillin-resistant Staphylococcus aureus (MRSA). No decrease in MRSA susceptibility was observed after 25 days of sub-lethal exposure to these AMPs. The AMPs bound preferentially to bacterial membrane lipids and induced membrane permeability leading to cytoplasmic leakage. Topical administration of Lynronne-1 (10% w/v) to a mouse model of MRSA wound infection elicited a significant reduction in bacterial counts, which was comparable to treatment with 2% mupirocin ointment. Our findings indicate that the rumen microbiome may provide viable alternative antimicrobials for future therapeutic application.

U2 - 10.1038/s41522-017-0042-1

DO - 10.1038/s41522-017-0042-1

M3 - Article

VL - 3

JO - NPJ Biofilms and Microbiomes

JF - NPJ Biofilms and Microbiomes

SN - 2055-5008

M1 - 33

ER -