Hybrid orthogonal frequency division multiplexing-digital filter multiple access passive optical networks (OFDM-DFMA PONs) offer a cost-effective solution to the challenging requirements of next-generation optical access networks and 5G and beyond radio access networks. It is crucial to consider the impact of timing jitter in any ADC/DAC-based system, therefore this paper presents an in-depth investigation into the impacts of DAC/ADC timing jitter on the hybrid OFDM-DFMA PON’s performance. We introduce improved accuracy white and coloured, DAC and ADC timing jitter models, applicable to any DSP-based transmission system. We prove that DAC and ADC timing jitter effects are virtually identical and investigate the effects of white/coloured timing jitter on upstream performance in hybrid OFDM-DFMA PONs and determine the associated jitter-induced optical power penalties. To mitigate against the timing jitter-induced performance degradations, a simple, but highly effective DSP-based technique is implemented which increases robustness against the timing jitter effects and significantly reduces timing jitter-induced optical power penalties. This consequently relaxes DAC/ADC sampling clock jitter requirements and so reduces implementation costs. White (coloured) timing jitter effects are shown to be independent of (dependent on) ONU operating frequency band and a trade-off between DAC and ADC jitter levels can be exploited to reduce ONU costs.