Intraspecific selection of Bacillus thuringiensis strains producing extracellular alkaline ribonucleases was carried out. Subtoxicus subspecies with increased expression of the enzyme was detected. A method was developed to isolate preparative amounts of homogeneous extracellular RNase of B. thuringiensis var. subtoxicus. The physico-chemical and catalytic properties of the enzyme was studied and compared with extracellular RNases of others Bacillus species. The conclusion about the structural and evolutional conservation of Bacillus extracellular RNases was drawn.

Keywords

  • Amino Acid Sequence, Amino Acids/analysis, Bacillus thuringiensis/enzymology, Calorimetry, Catalysis, Chromatography, High Pressure Liquid, Electrophoresis, Polyacrylamide Gel, Mass Spectrometry, Molecular Sequence Data, Ribonucleases/chemistry
Original languageRussian
Pages (from-to)1338-49
Number of pages12
JournalMolekuliarnaia biologiia
Volume26
Issue number6
Publication statusPublished - 1 Nov 1992
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