Vnekletochnaia shchelochnaia ribonukleaza Bacillus thuringiensis var. subtoxicus
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In: Molekuliarnaia biologiia, Vol. 26, No. 6, 01.11.1992, p. 1338-49.
Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Vnekletochnaia shchelochnaia ribonukleaza Bacillus thuringiensis var. subtoxicus
AU - Dement'ev, A A
AU - Riabchenko, N F
AU - Protasevich, I I
AU - Golyshin, P N
AU - Stepanov, A I
AU - Orlov, V M
AU - Pustovaev, V N
AU - Makarov, A A
AU - Moiseev, G P
AU - Karpeĭskiĭ, M Ia
PY - 1992/11/1
Y1 - 1992/11/1
N2 - Intraspecific selection of Bacillus thuringiensis strains producing extracellular alkaline ribonucleases was carried out. Subtoxicus subspecies with increased expression of the enzyme was detected. A method was developed to isolate preparative amounts of homogeneous extracellular RNase of B. thuringiensis var. subtoxicus. The physico-chemical and catalytic properties of the enzyme was studied and compared with extracellular RNases of others Bacillus species. The conclusion about the structural and evolutional conservation of Bacillus extracellular RNases was drawn.
AB - Intraspecific selection of Bacillus thuringiensis strains producing extracellular alkaline ribonucleases was carried out. Subtoxicus subspecies with increased expression of the enzyme was detected. A method was developed to isolate preparative amounts of homogeneous extracellular RNase of B. thuringiensis var. subtoxicus. The physico-chemical and catalytic properties of the enzyme was studied and compared with extracellular RNases of others Bacillus species. The conclusion about the structural and evolutional conservation of Bacillus extracellular RNases was drawn.
KW - Amino Acid Sequence
KW - Amino Acids/analysis
KW - Bacillus thuringiensis/enzymology
KW - Calorimetry
KW - Catalysis
KW - Chromatography, High Pressure Liquid
KW - Electrophoresis, Polyacrylamide Gel
KW - Mass Spectrometry
KW - Molecular Sequence Data
KW - Ribonucleases/chemistry
M3 - Erthygl
C2 - 1491677
VL - 26
SP - 1338
EP - 1349
JO - Molekuliarnaia biologiia
JF - Molekuliarnaia biologiia
SN - 0026-8984
IS - 6
ER -