Activity screening of environmental metagenomic libraries reveals novel carboxylesterase families

Popovic, A., Hai, T., Tchigvintsev, A., Hajighasemi, M., Nocek, B., Khusnutdinova, A. N., Brown, G., Glinos, J., Flick, R., Skarina, T., Chernikova, T., Yim, V., Bruls, T., Le Paslier, D., Yakimov, M. M., Joachimiak, A., Ferrer, M., Golyshina, O., Savchenko, A., Golyshin, P. & Yakunin, A. F., Mar 2017, In: Scientific Reports. 7, 44103, 44103.

Research output: Contribution to journal › Article › peer-review

Alcalilimnicola halodurans gen. nov., sp nov., an alkaliphilic, moderately halophilic and extremely halotolerant bacterium, isolated from sediments of soda-depositing Lake Natron, East Africa Rift Valley.

Yakimov, M. M., Giuliano, L., Chernikova, T. N., Gentile, G., Abraham, W. R., Lunsdorf, H., Timmis, K. N. & Golyshin, P. N., 1 Nov 2001, In: International Journal of Systematic and Evolutionary Microbiology. 51, 6, p. 2133-2143

Research output: Contribution to journal › Article › peer-review

Bacterial population and biodegradation potential in chronically crude oil-contaminated marine sediments are strongly linked to temperature

Bargiela, R., Mapelli, F., Rojo, D., Chouaia, B., Tornés, J., Borin, S., Richter, M., Del Pozo, M. V., Cappello, S., Gertler, C., Genovese, M., Denaro, R., Martínez-Martínez, M., Fodelianakis, S., Amer, R. A., Bigazzi, D., Han, X., Chen, J., Chernikova, T. N., Golyshina, O. V., Mahjoubi, M., Jaouanil, A., Benzha, F., Magagnini, M., Hussein, E., Al-Horani, F., Cherif. A., [. V., Blaghen, M., Abdel-Fattah, Y. R., Kalogerakis, N., Barbas, C., Malkawi, H. I., Golyshin, P. N., Yakimov, M. M., Daffonchio, D. & Ferrer, M., 29 Jun 2015, In: Scientific Reports. 5, p. Article Number 11651

Research output: Contribution to journal › Article › peer-review

Biochemical and structural features of a novel cyclodextrinase from cow rumen metagenome.

Ferrer, M., Beloqui, A., Golyshina, O. V., Plou, F. J., Neef, A., Chernikova, T. N., Fernandez-Arrojo, L., Ghazi, I., Ballesteros, A., Elborough, K., Timmis, K. N. & Golyshin, P. N., 1 Jan 2007, In: Biotechnology Journal. 2, 2, p. 207-213

Research output: Contribution to journal › Article › peer-review

Bioprospecting reveals class III ω-transaminases converting bulky ketones and environmentally relevant polyamines

Coscolín, C., Katzke, N., Garcia-Moyano, A., Navarro-Fernández, J., Almendral, D., Martinez-Martinez, M., Bollinger, A., Bargiela, R., Gerler, C., Chernikova, T. N., Rojo, D., Barbas, C., Tran, H., Golyshina, O. V., Koch, R., Yakimov, M. M., Bjerga, G. E. K., Golyshin, P. N., Jaeger, K.-E. & Ferrer, M., 9 Jan 2019, In: Applied and Environmental Microbiology. 85, 2, e02404-18.

Research output: Contribution to journal › Article › peer-review

Chaperonins govern growth of Escherichia coli at low temperatures.

Ferrer, M., Chernikova, T. N., Yakimov, M. M., Golyshin, P. N. & Timmis, K. N., 1 Nov 2003, In: Nature Biotechnology. 21, 11, p. 1266-1267

Research output: Contribution to journal › Article › peer-review

Determinants and prediction of esterase substrate promiscuity patterns

Martínez-Martínez, M., Coscolín, C., Santiago, G., Chow, J., Stogios, P. J., Bargiela, R., Gertler, C., Navarro-Fernández, J., Bollinger, A., Thies, S., Méndez-García, C., Popovic, A., Brown, G., Chernikova, T., García-Moyano, A., Bjerga, G. E. K., Perez-Garcia, P., Hai, T., del Pozo, M. V., Stokke, R., Steen, I. H., Cui, H., Xu, X., Nocek, B., Alcaide, M., Disasto, M., Mesa, V., Pelaez, A. I., Sanchez, J., Buchholz, P. C. F., Pleiss, J., Fernández-Guerra, A., Glockner, F. O., Golyshina, O., Yakimov, M. M., Savchenko, A., Jaeger, K.-E., Yakunin, A. F., Streit, W. R., Golyshin, P., Guallar, V. & Ferrer, M., 2018, In: ACS Chemical Biology. 13, 1, p. 225-234

Research output: Contribution to journal › Article › peer-review

Diversity of hydrolases from hydrothermal vent sediments of the Levante Bay, Vulcano Island (Aeolian archipelago) identified by activity-based metagenomics and biochemical characterization of new esterases and an arabinopyranosidase

Placido, A., Hai, T., Ferrer, M., Chernikova, T. N., Distaso, M. A., Armstrong, D., Yakunin, A. F., Toshchakov, S. V., Yakimov, M. M., Kublanov, I. V., Golyshina, O., Pesole, G., Ceci, L. R. & Golyshin, P., 1 Dec 2015, In: Applied Microbiology and Biotechnology. 99, 23, p. 10031-10046

Research output: Contribution to journal › Article › peer-review

Enzyme adaptation to habitat thermal legacy shapes the thermal plasticity of marine microbiomes

Marasco, R., Fusi, M., Coscolín, C., Borozzi, A., Almendral, D., Bargiela, R., Gohlke neé Nutschel, C., Dittrich, J., Gohlke, H., Matesanz, R., Sanchez-Carrillo, S., Mapelli, F., Chernikova, T., Golyshin, P., Ferrer, M. & Daffonchio, D., 24 Feb 2023, In: Nature Communications. 14, 1, 1045.

Research output: Contribution to journal › Article › peer-review

Expression of a temperature-sensitive esterase in anovel chaperone-based Escherichia coli strain.

Ferrer, M., Chernikova, T. N., Timmis, K. N. & Golyshin, P. N., 1 Aug 2004, In: Applied and Environmental Microbiology. 70, 8, p. 4499-4504

Research output: Contribution to journal › Article › peer-review

Functional Metagenomics Unveils a Multifunctional Glycosyl Hydrolase from the Family 43 Catalysing the Breakdown of Plant Polymers in the Calf Rumen

Ferrer, M., Ghazi, A., Beloqui, A., Vieites, J. M., Lopez-Cortes, N., Marın-Navarro, J., Nechitaylo, T. Y., Guazzaroni, M. E., Polaina, J., Waliczek, A., Chernikova, T. N., Reva, O. N., Golyshina, O. V. & Golyshin, P. N., 25 Jun 2012, In: PLoS ONE. 7, 6, p. e38134

Research output: Contribution to journal › Article › peer-review

Functional consequences of single:double ring transitions in chaperonins: life in the cold.

Ferrer, M., Lunsdorf, H., Chernikova, T. N., Yakimov, M., Timmis, K. N. & Golyshin, P. N., 1 Jul 2004, In: Molecular Microbiology. 53, 1, p. 167-182

Research output: Contribution to journal › Article › peer-review

Genome Sequence of Thalassolituus oleivorans MIL-1 (DSM 14913T)

Hai, T., Golyshin, P. N., Werner, J., Chernikova, T. N., Tran, H., Ferrer, M., Yakimov, M. M., Teeling, H. & Golyshina, O. V., 18 Apr 2013, In: Genome Announcements. 1, 2, p. e00141-13

Research output: Contribution to journal › Article › peer-review

Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica

Golyshina, O., Hai, T., Kube, M., Chernikova, T. N., Al-Ramahi, Y., Beloqui, A., Lopez-Cortez, N., Guazzaroni, M. E., Heipieper, H. J., Klages, S., Kotsyurbenko, O. R., Langer, I., Nechitaylo, T. Y., Lünsdorf, H., Fernández, M., Juárez, S., Ciordia, S., Singer, A., Kagan, O., Egorova, O., Petit, P. A., Stogios, P., Kim, Y., Tchigvintsev, A., Flick, R., Denaro, R., Genovese, M., Albar, J. P., Reva, O. N., Martínez-Gomariz, M., Tran, H., Ferrer, M., Savchenko, A., Yakunin, A. F., Yakimov, M. M., Golyshina, O. V., Reinhardt, R. & Golyshin, P. N., 23 Jul 2013, In: Nature Communications. 4, p. 2156

Research output: Contribution to journal › Article › peer-review

Genome sequence completed of Alcanivorax borkumensis, a hydrocarbon-degrading bacterium that plays a global role in oil removal from marine systems.

Golyshin, P. N., Dos Santos, V. A., Kaiser, O., Ferrer, M., Sabirova, Y. S., Lunsdorf, H., Chernikova, T. N., Golyshina, O. V., Yakimov, M. M., Puhler, A. & Timmis, K. N., 19 Dec 2003, In: Journal of Biotechnology. 106, 2-3, p. 215-220

Research output: Contribution to journal › Article › peer-review

Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis.

Schneiker, S., Martins dos Santos, V. A. P., Bartels, D., Bekel, T., Brecht, M., Buhrmester, J., Chernikova, T. N., Denaro, R., Ferrer, M., Gertler, C., Goesmann, A., Golyshina, O. V., Kaminski, F., Khachane, A. N., Lang, S., Linke, B., McHardy, A. C., Meyer, F., Nechitaylo, T., Pühler, A., Regenhardt, D., Rupp, O., Sabirova, J. S., Selbitschka, W., Yakimov, M. M., Timmis, K. N., Vorhölter, F.-J., Weidner, S., Kaiser, O. & Golyshin, P. N., 30 Jul 2006, In: Nature Biotechnology. 24, 8, p. 997-1004 8 p.

Research output: Contribution to journal › Article › peer-review

Hydrocarbon-Degrading Bacteria Alcanivorax and Marinobacter Associated With Microalgae Pavlova lutheri and Nannochloropsis oculata

Chernikova, T., Bargiela, R., Toshchakov, S. V., Shivaraman, V., Lunev, E. A., Yakimov, M. M., Thomas, D. N. & Golyshin, P., 28 Oct 2020, In: Frontiers in Microbiology. 11, 572931.

Research output: Contribution to journal › Article › peer-review

Insights into the degradation capacities of Amycolatopsis tucumanensis DSM 45259 guided by microarray data

Bourguignon, N., Bargiela, R., Rojo, D., Chernikova, T., Lopez de Rodas, S. A., Garcia-Cantalejo, J., Nather, D. J., Golyshin, P., Barbas, C., Ferrero, M. & Ferrer, M., 1 Dec 2016, In: World Journal of Microbiology and Biotechnology. 32, 201

Research output: Contribution to journal › Article › peer-review

Metaproteomics and metabolomics analyses of chronically petroleum-polluted sites reveal the importance of general anaerobic processes uncoupled with degradation

Bargiela, R., Herbst, F. A., Martinez-Martinez, M., Seifert, J., Rojo, D., Cappello, S., Genovese, M., Crisafi, F., Denaro, R., Chernikova, T. N., Barbas, C., von Bergen, M., Yakimov, M. M., Ferrer, M. & Golyshin, P. N., 27 Aug 2015, In: Proteomics. 15, 20, p. 3508-3520

Research output: Contribution to journal › Article › peer-review

Microbial communities colonising plastics during transition from the wastewater treatment plant to marine waters

Tulloch, C. L., Bargiela, R., Williams, G. B., Chernikova, T. N., Cotterell, B. M., Wellington, E. M. H., Christie-Oleza, J., Thomas, D. N., Jones, D. L. & Golyshin, P. N., 29 Apr 2024, In: Environmental Microbiome. 19, 1, 27.

Research output: Contribution to journal › Article › peer-review

Microbial community of a saline mud volcano at San Biagio-Belpasso, Mt. Etna (Italy)

Yakimov, M. M., Giuliano, L., Crisafi, E., Chernikova, T. N., Timmis, K. N. & Golyshin, P. N., 1 May 2002, In: Environmental Microbiology. 4, 5, p. 249-256

Research output: Contribution to journal › Article › peer-review

Microbial enzymes mined from the Urania deep-sea hypersaline anoxic basin.

Ferrer, M., Golyshina, O. V., Chernikova, T. N., Khachane, A. N., Dos Santos, V. A., Yakimov, M. M., Timmis, K. N. & Golyshin, P. N., 1 Aug 2005, In: Chemistry and Biology. 12, 8, p. 895-904

Research output: Contribution to journal › Article › peer-review

Microbial β-glucosidases from cow rumen metagenome enhance the saccharification of lignocellulose in combination with commercial cellulase cocktail

Del Pozo, M. V., Fernández-Arrojo, L., Gil-Martínez, J., Montesinos, A., Chernikova, T. N., Nechitaylo, T. Y., Waliszek, A., Tortajada, M., Rojas, A., Huws, S. A., Golyshina, O., Newbold, C. M., Polaina, J., Ferrer, M. & Golyshin, P., 12 Sept 2012, In: Biotechnology for Biofuels. 5, p. 73

Research output: Contribution to journal › Article › peer-review

Monaibacterium marinum, gen. nov, sp. nov, a new member of the Alphaproteobacteria isolated from seawater of Menai Straits, Wales, UK

Chernikova, T., Dallimore, J., Lunsdorf, H., Heipieper, H. J. & Golyshin, P., 1 Sept 2017, In: International Journal of Systematic Evolutionary Microbiology. 67, p. 3310-3317

Research output: Contribution to journal › Article › peer-review

Natural microbial diversity in superficial sediments of Milazzo Harbor (Sicily) and community successions during microcosm enrichment with various hydrocarbons.

Yakimov, M. M., Denaro, R., Genovese, M., Cappello, S., D'Auria, G., Chernikova, T. N., Timmis, K. N., Golyshin, P. N. & Giuliano, L., 1 Sept 2005, In: Environmental Microbiology. 7, 9, p. 1426-1441

Research output: Contribution to journal › Article › peer-review

Niche-specificity factors of a marine oil-degrading bacterium Alcanivorax borkumensis SK2.

Sabirova, J. S., Chernikova, T. N., Timmis, K. N. & Golyshin, P. N., 1 Aug 2008, In: FEMS Microbiology Letters. 285, 1, p. 89-96

Research output: Contribution to journal › Article › peer-review

Novel Hybrid Esterase-Haloacid Dehalogenase Enzyme

Beloqui, A., Polaina, J., Maria Vieites, J., Reyes-Duarte, D., Torres, R., Golyshina, O. V., Chernikova, T. N., Waliczek, A., Aharoni, A., Yakimov, M. M., Timmis, K. N., Golyshin, P. N. & Ferrer, M., 16 Aug 2010, In: ChemBioChem. 11, 14, p. 1975-1978

Research output: Contribution to journal › Article › peer-review

Novel hydrolase diversity retrieved from a metagenome library of bovine rumen microflora.

Ferrer, M., Golyshina, O. V., Chernikova, T. N., Khachane, A. N., Reyes-Duarte, D., Dos Santos, V. A., Strompl, C., Elborough, K., Jarvis, G., Neef, A., Yakimov, M. M., Timmis, K. N. & Golyshin, P. N., 1 Dec 2005, In: Environmental Microbiology. 7, 12, p. 1996-2010

Research output: Contribution to journal › Article › peer-review

Oleiphilaceae fam. nov., to include Oleiphilus messinensis gen. nov., sp nov., a novel marine bacterium that obligately utilizes hydrocarbons.

Golyshin, P. N., Chernikova, T. N., Abraham, W. R., Lunsdorf, H., Timmis, K. N. & Yakimov, M. M., 1 May 2002, In: International Journal of Systematic and Evolutionary Microbiology. 52, 3, p. 901-911

Research output: Contribution to journal › Article › peer-review

Oleispira antarctica gen. nov., sp nov., a novel hydrocarbonoclastic marine bacterium isolated from Antarctic coastal sea water.

Yakimov, M. M., Giuliano, L., Gentile, G., Crisafi, E., Chernikova, T. N., Abraham, W. R., Lunsdorf, H., Timmis, K. N. & Golyshin, P. N., 1 May 2003, In: International Journal of Systematic and Evolutionary Microbiology. 53, 3, p. 779-785

Research output: Contribution to journal › Article › peer-review

Polyesterase activity is widespread in the family IV carboxylesterases from bacteria

Ma, H., Khusnutdinova, A. N., Lemak, S., Chernikova, T. N., Golyshina, O. V., Almendral, D., Ferrer, M., Golyshin, P. N. & Yakunin, A. F., 5 Jan 2025, In: Journal of Hazardous Materials. 481, p. 136540

Research output: Contribution to journal › Article › peer-review

Pressure adaptation is linked to thermal adaptation in salt-saturated marine habitats

Alcaide, M., Stogios, P. J., Lafraya, A., Tchigvintsev, A., Flick, R., Bargiela, R., Chernikova, T. N., Reva, O. N., Hai, T., Leggewie, C. C., Katzke, N., La Cono, V., Matesanz, R., Jebbar, M., Jaeger, K., Yakimov, M. M., Yakunin, A. F., Golyshin, P. N., Golyshina, O. V., Savchenko, A. & Ferrer, M., 17 Dec 2014, In: Environmental Microbiology. 17, 2, p. 332-345

Research output: Contribution to journal › Article › peer-review

Prevalence of integrons in multidrug‑resistant Escherichia coli isolates from waters and vegetables in Nsukka and Enugu, Southeast Nigeria

Chigor, C. B., Ibangha, I.-A. I., Nweze, N. O., Onuora, V. C., Ozochi, C. A., Titilawo, Y., Enebe, M. C., Chernikova, T., Golyshin, P. & Chigor, V. N., Aug 2022, In: Environmental Science and Pollution Research. 29, 40, p. 60945-60952

Research output: Contribution to journal › Article › peer-review

Screening and Characterization of Novel Polyesterases from Environmental Metagenomes with High Hydrolytic Activity against Synthetic Polyesters

Hajighasemi, M., Tchigvintsev, A., Nocek, B., Flick, R., Popovic, A., Hai, T., Khusnutdinova, A. N., Brown, G., Xu, X., Cui, H., Anstett, J., Chernikova, T., Bruls, T., Le Paslier, D., Yakimov, M. M., Joachimiak, A., Golyshina, O., Savchenko, A., Golyshin, P., Edwards, E. A. & Yakunin, A. F., 6 Nov 2018, In: Environmental Science and Technology. 52, 21, p. 12388-12401

Research output: Contribution to journal › Article › peer-review

Single residues dictate the co-evolution of dual esterases: MCP hydrolases from the α/β hydrolase family

Alcaide, M., Tornés, J., Stogios, P. J., Xu, X., Gertler, C., Di Leo, R., Bargiela, R., Lafraya, A., Guazzaroni, M. E., López‑Cortés, N., Chernikova, T. N., Golyshina, O. V., Nechitaylo, T. Y., Plumeier∥, I., Pieper∥, D. H., Yakimov, M. M., Savchenko, A., Golyshin, P. N. & Ferrer, M., 15 Aug 2013, In: Biochemical Journal. 454, 1, p. 157-166

Research output: Contribution to journal › Article › peer-review

Structure and activity of the cold-active and anion-activated carboxyl esterase OLEI01171 from the oil-degrading marine bacterium Oleispira antarctica

Lemak, S., Tchigvintsev, A., Petit, P., Flick, R., Singer, A. U., Brown, G., Evdokimova, E., Egorova, O., Gonzalez, C. F., Chernikova, T. N., Yakimov, M. M., Kube, M., Reinhardt, R., Golyshin, P. N., Savchenko, A. & Yakunin, A. F., 15 Jul 2012, In: Biochemical Journal. 445, 2, p. 193-203

Research output: Contribution to journal › Article › peer-review

Thalassolituus oleivorans gen. nov., sp nov., a novel marine bacterium that obligately utilizes hydrocarbons

Yakimov, M. M., Giuliano, L., Denaro, R., Crisafi, E., Chernikova, T. N., Abraham, W. R., Luensdorf, H., Timmis, K. N. & Golyshin, P. N., 1 Jan 2004, In: International Journal of Systematic and Evolutionary Microbiology. 54, 1, p. 141-148

Research output: Contribution to journal › Article › peer-review

The Mobility of the Cap Domain Is Essential for the Substrate Promiscuity of a Family IV Esterase from Sorghum Rhizosphere Microbiome

Distaso, M., Cea-Rama, I., Coscolín, C., Chernikova, T., Tran, H., Ferrer, M., Sanz-Aparico, J. & Golyshin, P., 31 Jan 2023, In: Applied and Environmental Microbiology. 89, 1, p. e0180722 e0180722.

Research output: Contribution to journal › Article › peer-review

The environment shapes microbial enzymes: five cold-active and salt-resistant carboxylesterases from marine metagenomes

Tchigvintsev, A., Tran, H., Popvic, A., Kovacic, F., Brown, G., Flick, R., Hajighasemi, M., Egorova, O., Somody, J. C., Tchigvintsev, D., Khusnutdinova, A., Chernikova, T. N., Golyshina, O., Yakimov, M. M., Savchenko, A., Golyshin, P., Jaeger, K. E. & Yakunin, A. F., 1 Mar 2015, In: Applied Microbiology and Biotechnology. 99, 5, p. 2165-2178

Research output: Contribution to journal › Article › peer-review

The genome analysis of Oleiphilus messinensis ME102 (DSM 13489T) reveals backgrounds of its obligate alkane-devouring marine lifestyle

Toshchakov, S. V., Korzhenkov, A. A., Chernikova, T., Ferrer, M., Golyshina, O., Yakimov, M. M. & Golyshin, P., Dec 2017, In: Marine Genomics. 36, p. 41-47

Research output: Contribution to journal › Article › peer-review

Thermophilic Carboxylesterases from Hydrothermal Vents of the Volcanic Island of Ischia Active on Synthetic and Biobased Polymers and Mycotoxins

Distaso, M., Chernikova, T., Bargiela, R., Coscolín, C., Stogios, P. J., Gonzalez-Alfonso, J. L., Lemak, S., Khusnutdinova, A., Plou, F. J., Evdokimova, E., Savchenko, A., Lunev, E., Yakimov, M. M., Golyshina, O., Ferrer, M., Yakunin, A. & Golyshin, P., 28 Feb 2023, In: Applied and Environmental Microbiology. 89, 2, p. e0170422 20 p.

Research output: Contribution to journal › Article › peer-review

Unveiling microbial life in the new deep-sea hypersaline Lake Thetis. Part II: a metagenomic study

Ferrer, M., Werner, J., Chernikova, T. N., Bargiela, R., Fernández, L. L., Waldmann, J., Teeling, H., Golyshina, O. V., Glöckner, F. O., Yakimov, M. M. & Golyshin, P. N., 1 Jan 2012, In: Environmental Microbiology. 14, 1, p. 268-281

Research output: Contribution to journal › Article › peer-review

Utilization of low-molecular-weight organic compounds by the filterable fraction of a lotic microbiome

Ghuneim, L. A., Distaso, M., Chernikova, T., Bargiela, R., Lunev, E. A., Korzhenkov, A. A., Toshchakov, S. V., Rojo, D., Barbas, C., Ferrer, M., Golyshina, O., Golyshin, P. & Jones, D. L., 1 Feb 2021, In: Fems Microbiology Ecology. 97, 2

Research output: Contribution to journal › Article › peer-review

Multidrug Resistance and High Prevalence of class 1 Integrons in Escherichia coli Isolated from Irrigation Water and Vegetables in Parts of Nsukka and Enugu, Nigeria

Chigor, C. B., Ibangha, I.-A. I., Onuora, V. C., Omotosho, O. E., Chernikova, T., Chigor, V. N. & Golyshin, P., 1 Apr 2017, In: Faseb Journal. 31, S1, 777.22.

Research output: Contribution to journal › Meeting Abstract › peer-review