“ARMAN” archaea depend on association with euryarchaeal host in culture and in situ

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  • Olga Golyshina
  • Stepan V. Toshchakov
    Immanuel Kant Baltic Federal University, Kaliningrad.
  • Kira S. Makarova
    National Institutes of Health
  • Sergei N. Gavrilov
    Russian Academy of Sciences
  • Alexei A. Korzhenkov
    Immanuel Kant Baltic Federal University, Kaliningrad.
  • Violetta La Cono
    Institute for Coastal Marine Environment
  • Erika Arcadi
    Institute for Coastal Marine Environment
  • Taras Y. Nechitaylo
    Max Planck Institute for Chemical Ecology, Jena.
  • Manuel Ferrer
    CSIC, Institute of Catalysis, Madrid
  • Ilya V. Kublanov
    Russian Academy of Sciences
  • Yuri I. Wolf
    National Institutes of Health
  • Michail M. Yakimov
    Institute for Coastal Marine Environment
  • Peter Golyshin
Intriguing, yet uncultured ‘ARMAN’-like archaea are metabolically dependent on other members of the microbial community. It remains uncertain though which hosts they rely upon, and, because of the lack of complete genomes, to what extent. Here, we report the co-culturing of ARMAN-2-related organism, Mia14, with Cuniculiplasma divulgatum PM4 during the isolation of this strain from acidic streamer in Parys Mountain (Isle of Anglesey, UK). Mia14 is highly enriched in the binary culture (ca. 10% genomic reads) and its ungapped 0.95 Mbp genome points at severe voids in central metabolic pathways, indicating dependence on the host, C. divulgatum PM4. Analysis of C. divulgatum isolates from different sites and shotgun sequence data of Parys Mountain samples suggests an extensive genetic exchange between Mia14 and hosts in situ. Within the subset of organisms with high-quality genomic assemblies representing the ‘DPANN’ superphylum, the Mia14 lineage has had the largest gene flux, with dozens of genes gained that are implicated in the host interaction.
Original languageEnglish
Article number60
JournalNature Communications
Early online date5 Jul 2017
Publication statusPublished - 2017

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