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“ARMAN” archaea depend on association with euryarchaeal host in culture and in situ

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“ARMAN” archaea depend on association with euryarchaeal host in culture and in situ. / Golyshina, Olga; Toshchakov, Stepan V.; Makarova, Kira S. et al.
In: Nature Communications, Vol. 8, 60, 2017.

Research output: Contribution to journal › Article › peer-review

HarvardHarvard

Golyshina, O, Toshchakov, SV, Makarova, KS, Gavrilov, SN, Korzhenkov, AA, La Cono, V, Arcadi, E, Nechitaylo, TY, Ferrer, M, Kublanov, IV, Wolf, YI, Yakimov, MM & Golyshin, P 2017, '“ARMAN” archaea depend on association with euryarchaeal host in culture and in situ', Nature Communications, vol. 8, 60. https://doi.org/10.1038/s41467-017-00104-7

APA

Golyshina, O., Toshchakov, S. V., Makarova, K. S., Gavrilov, S. N., Korzhenkov, A. A., La Cono, V., Arcadi, E., Nechitaylo, T. Y., Ferrer, M., Kublanov, I. V., Wolf, Y. I., Yakimov, M. M., & Golyshin, P. (2017). “ARMAN” archaea depend on association with euryarchaeal host in culture and in situ. Nature Communications, 8, Article 60. https://doi.org/10.1038/s41467-017-00104-7

CBE

Golyshina O, Toshchakov SV, Makarova KS, Gavrilov SN, Korzhenkov AA, La Cono V, Arcadi E, Nechitaylo TY, Ferrer M, Kublanov IV, et al. 2017. “ARMAN” archaea depend on association with euryarchaeal host in culture and in situ. Nature Communications. 8:Article 60. https://doi.org/10.1038/s41467-017-00104-7

MLA

Golyshina, Olga et al. "“ARMAN” archaea depend on association with euryarchaeal host in culture and in situ". Nature Communications. 2017. 8. https://doi.org/10.1038/s41467-017-00104-7

VancouverVancouver

Golyshina O, Toshchakov SV, Makarova KS, Gavrilov SN, Korzhenkov AA, La Cono V et al. “ARMAN” archaea depend on association with euryarchaeal host in culture and in situ. Nature Communications. 2017;8:60. Epub 2017 Jul 5. doi: 10.1038/s41467-017-00104-7

Author

Golyshina, Olga ; Toshchakov, Stepan V. ; Makarova, Kira S. et al. / “ARMAN” archaea depend on association with euryarchaeal host in culture and in situ. In: Nature Communications. 2017 ; Vol. 8.

RIS

TY - JOUR

T1 - “ARMAN” archaea depend on association with euryarchaeal host in culture and in situ

AU - Golyshina, Olga

AU - Toshchakov, Stepan V.

AU - Makarova, Kira S.

AU - Gavrilov, Sergei N.

AU - Korzhenkov, Alexei A.

AU - La Cono, Violetta

AU - Arcadi, Erika

AU - Nechitaylo, Taras Y.

AU - Ferrer, Manuel

AU - Kublanov, Ilya V.

AU - Wolf, Yuri I.

AU - Yakimov, Michail M.

AU - Golyshin, Peter

PY - 2017

Y1 - 2017

N2 - Intriguing, yet uncultured ‘ARMAN’-like archaea are metabolically dependent on other members of the microbial community. It remains uncertain though which hosts they rely upon, and, because of the lack of complete genomes, to what extent. Here, we report the co-culturing of ARMAN-2-related organism, Mia14, with Cuniculiplasma divulgatum PM4 during the isolation of this strain from acidic streamer in Parys Mountain (Isle of Anglesey, UK). Mia14 is highly enriched in the binary culture (ca. 10% genomic reads) and its ungapped 0.95 Mbp genome points at severe voids in central metabolic pathways, indicating dependence on the host, C. divulgatum PM4. Analysis of C. divulgatum isolates from different sites and shotgun sequence data of Parys Mountain samples suggests an extensive genetic exchange between Mia14 and hosts in situ. Within the subset of organisms with high-quality genomic assemblies representing the ‘DPANN’ superphylum, the Mia14 lineage has had the largest gene flux, with dozens of genes gained that are implicated in the host interaction.

AB - Intriguing, yet uncultured ‘ARMAN’-like archaea are metabolically dependent on other members of the microbial community. It remains uncertain though which hosts they rely upon, and, because of the lack of complete genomes, to what extent. Here, we report the co-culturing of ARMAN-2-related organism, Mia14, with Cuniculiplasma divulgatum PM4 during the isolation of this strain from acidic streamer in Parys Mountain (Isle of Anglesey, UK). Mia14 is highly enriched in the binary culture (ca. 10% genomic reads) and its ungapped 0.95 Mbp genome points at severe voids in central metabolic pathways, indicating dependence on the host, C. divulgatum PM4. Analysis of C. divulgatum isolates from different sites and shotgun sequence data of Parys Mountain samples suggests an extensive genetic exchange between Mia14 and hosts in situ. Within the subset of organisms with high-quality genomic assemblies representing the ‘DPANN’ superphylum, the Mia14 lineage has had the largest gene flux, with dozens of genes gained that are implicated in the host interaction.

U2 - 10.1038/s41467-017-00104-7

DO - 10.1038/s41467-017-00104-7

M3 - Article

VL - 8

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 60

ER -