A microplate screen to estimate metal-binding affinities of metalloproteins

Diep, P., Mahadevan, R. & Yakunin, A. F., 15 Nov 2020, In: Analytical biochemistry. 609, p. 113836

Research output: Contribution to journal › Article › peer-review

A random mutagenesis screen enriched for missense mutations in bacterial effector proteins

Urbanus, M. L., Zheng, T. M., Khusnutdinova, A. N., Banh, D., O'Connor Mount, H., Gupta, A., Stogios, P. J., Savchenko, A., Isberg, R. R., Yakunin, A. F. & Ensminger, A. W., 4 Sept 2024, In: G3: Genes, Genomes, Genetics. 14, 9

Research output: Contribution to journal › Article › peer-review

Activity screening of environmental metagenomic libraries reveals novel carboxylesterase families

Popovic, A., Hai, T., Tchigvintsev, A., Hajighasemi, M., Nocek, B., Khusnutdinova, A. N., Brown, G., Glinos, J., Flick, R., Skarina, T., Chernikova, T., Yim, V., Bruls, T., Le Paslier, D., Yakimov, M. M., Joachimiak, A., Ferrer, M., Golyshina, O., Savchenko, A., Golyshin, P. & Yakunin, A. F., Mar 2017, In: Scientific Reports. 7, 44103, 44103.

Research output: Contribution to journal › Article › peer-review

An Amino Acid Self-Regenerating Cell-Free Protein Synthesis System that Feeds on PLA Plastics, CO2, Ammonium, and α-ketoglutarate

Nishikawa, S., Yu, W.-C., Jia, T. Z., He, M.-J., Khusnutdinova, A., Yakunin, A., Chiang, Y.-R., Fujishima, K. & Wang, P.-H., 17 May 2024, In: ACS Catalysis. 14, 10, p. 7696-7706

Research output: Contribution to journal › Article › peer-review

Biochemical and structural insights into enzymatic depolymerization of polylactic acid and other polyesters by microbial carboxylesterases

Hajighasemi, M., Nocek, B. P., Tchigvintsev, A., Brown, G., Flick, R., Xu, X., Cui, H., Hai, T., Joachimiak, A., Golyshin, P., Savchenko, A., Edwards, E. A. & Yakunin, A. F., 18 Apr 2016, In: Biomacromolecules. 17, 6, p. 2027-2039

Research output: Contribution to journal › Article › peer-review

Biochemical plasticity of the Escherichia coli CRISPR Cascade revealed by in vitro reconstitution of Cascade activities from purified Cas proteins

Lemak, S., Brown, G., Makarova, K. S., Koonin, E. V. & Yakunin, A., 2024, In: Febs Journal. p. 1-18 18 p.

Research output: Contribution to journal › Article › peer-review

CRISPR-induced DNA reorganization for multiplexed nucleic acid detection

Karlikow, M., Amalfitano, E., Yang, X., Doucet, J., Chapman, A., Sadat-Moussavi, P., Homme, P., Sutyrina, P., Chang, W., Lemak, S., Yakunin, A., Dolezal, A. G., Kelley, S., Foster, L., Harpur, B. & Pardee, K., 17 Mar 2023, In: Nature Communications. 14, 1, 1505.

Research output: Contribution to journal › Article › peer-review

Determinants and prediction of esterase substrate promiscuity patterns

Martínez-Martínez, M., Coscolín, C., Santiago, G., Chow, J., Stogios, P. J., Bargiela, R., Gertler, C., Navarro-Fernández, J., Bollinger, A., Thies, S., Méndez-García, C., Popovic, A., Brown, G., Chernikova, T., García-Moyano, A., Bjerga, G. E. K., Perez-Garcia, P., Hai, T., del Pozo, M. V., Stokke, R., Steen, I. H., Cui, H., Xu, X., Nocek, B., Alcaide, M., Disasto, M., Mesa, V., Pelaez, A. I., Sanchez, J., Buchholz, P. C. F., Pleiss, J., Fernández-Guerra, A., Glockner, F. O., Golyshina, O., Yakimov, M. M., Savchenko, A., Jaeger, K.-E., Yakunin, A. F., Streit, W. R., Golyshin, P., Guallar, V. & Ferrer, M., 2018, In: ACS Chemical Biology. 13, 1, p. 225-234

Research output: Contribution to journal › Article › peer-review

Diversity of hydrolases from hydrothermal vent sediments of the Levante Bay, Vulcano Island (Aeolian archipelago) identified by activity-based metagenomics and biochemical characterization of new esterases and an arabinopyranosidase

Placido, A., Hai, T., Ferrer, M., Chernikova, T. N., Distaso, M. A., Armstrong, D., Yakunin, A. F., Toshchakov, S. V., Yakimov, M. M., Kublanov, I. V., Golyshina, O., Pesole, G., Ceci, L. R. & Golyshin, P., 1 Dec 2015, In: Applied Microbiology and Biotechnology. 99, 23, p. 10031-10046

Research output: Contribution to journal › Article › peer-review

Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica

Golyshina, O., Hai, T., Kube, M., Chernikova, T. N., Al-Ramahi, Y., Beloqui, A., Lopez-Cortez, N., Guazzaroni, M. E., Heipieper, H. J., Klages, S., Kotsyurbenko, O. R., Langer, I., Nechitaylo, T. Y., Lünsdorf, H., Fernández, M., Juárez, S., Ciordia, S., Singer, A., Kagan, O., Egorova, O., Petit, P. A., Stogios, P., Kim, Y., Tchigvintsev, A., Flick, R., Denaro, R., Genovese, M., Albar, J. P., Reva, O. N., Martínez-Gomariz, M., Tran, H., Ferrer, M., Savchenko, A., Yakunin, A. F., Yakimov, M. M., Golyshina, O. V., Reinhardt, R. & Golyshin, P. N., 23 Jul 2013, In: Nature Communications. 4, p. 2156

Research output: Contribution to journal › Article › peer-review

Heavy metal removal by bioaccumulation using genetically engineered microorganisms

Diep, P., Mahadevan, R. & Yakunin, A., 29 Oct 2018, In: Frontiers in Bioengineering and Biotechnology. 6, 157.

Research output: Contribution to journal › Article › peer-review

Identification and characterization of carboxyl esterases of gill chamber-associated microbiota in the deep-sea shrimp Rimicaris exoculata using functional metagenomics

Alcaide, M., Tchigvinstsev, A., Martinez-Martinez, M., Popovic, A., Reva, O. N., Lafraya, A., Bargiela, R. N., Nechitaylo, T. Y., Matesanz, R., Cambon-Bonavita, M., Jebbar, M., Savchenko, A., Yakimov, M. M., Golyshina, O., Yakunin, A. F., Golyshin, P., Ferrer, M. & The MAMBA Consortium., [. V., 16 Jan 2015, In: Applied and Environmental Microbiology. 81, 6, p. 2125-2136

Research output: Contribution to journal › Article › peer-review

Lignin-oxidizing activity of bacterial laccases characterized using soluble substrates and polymeric lignin

Choolaei, Z., Flick, R., Khusnutdinova, A., Edwards, E. A. & Yakunin, A. F., 10 Jan 2021, In: Journal of Biotechnology. 325, p. 128-137 10 p.

Research output: Contribution to journal › Article › peer-review

Metabolic and evolutionary patterns in the extremely acidophilic archaeon Ferroplasma acidiphilum YT

Golyshina, O., Hai, T., Reva, O. N., Lemak, S., Yakunin, A. F., Goesmann, A., Nechitaylo, T. Y., LaCono, V., Smedile, F., Slesarev, A., Rojo, D., Barbas, C., Ferrer, M., Yakimov, M. M. & Golyshin, P., 16 Jun 2017, In: Scientific Reports. 7, 3682.

Research output: Contribution to journal › Article › peer-review

Metabolite Damage and Damage-Control in a Minimal Genome

Haas, D., Thamm, A. M., Sun, J., Huang, L., Sun, L., Beaudoin, G. A. W., Wise, K. S., Lema-Ortiz, C., Bruner, S. D., Breuer, M., Luthey-Schutten, Z., Lin, J., Wilson, M. A., Brown, G., Yakunin, A., Kurilyak, I., Folz, J., Fiehn, O., Glass, J. I., Hanson, A. D., Henry, C. S. & de Crecy-Lagard, V., 30 Aug 2022, In: mBio. e0163022.

Research output: Contribution to journal › Article › peer-review

Moderately thermostable GH1 β-glucosidases from hyperacidophilic archaeon Cuniculiplasma divulgatum S5

Khusnutdinova, A., Hai, T., Devlekar, S., Distaso, M., Kublanov, I. V., Skarina, T., Stogios, P., Savchenko, A., Ferrer, M., Golyshina, O., Yakunin, A. & Golyshin, P., Sept 2024, In: Fems Microbiology Ecology. 100, 9, fiae114.

Research output: Contribution to journal › Article › peer-review

Ni(II)-binding affinity of CcNikZ-II and its homologs: the role of the HH-prong and variable loop revealed by structural and mutational studies

Diep, P., Stogios, P. J., Evdokimova, E., Savchenko, A., Mahadevan, R. & Yakunin, A. F., Jul 2024, In: Febs Journal. 291, 13, p. 2980-2993 14 p.

Research output: Contribution to journal › Article › peer-review

One-pot biocatalytic transformation of adipic acid to 6-aminocaproic acid and 1,6-hexamethylenediamine using carboxylic acid reductases and transaminases

Fedorchuk, T. P., Khusnutdinova, A. N., Evdokimova, E., Flick, R., Di Leo, R., Stogios, P. J., Savchenko, A. & Yakunin, A., 15 Jan 2020, In: Journal of the American Chemical Society. 142, 2, p. 1038-1048 11 p.

Research output: Contribution to journal › Article › peer-review

One-pot chemo-enzymatic synthesis and one-step recovery of length-variable long-chain polyphosphates from microalgal biomass

Lin, Y.-H., Nishikawa, S., Jia, T. Z., Yeh, F.-I., Khusnutdinova, A., Yakunin, A., Fujishima, K. & Wang, P.-H., 27 Oct 2023, In: Green Chemistry. 23

Research output: Contribution to journal › Article › peer-review

Polyesterase activity is widespread in the family IV carboxylesterases from bacteria

Ma, H., Khusnutdinova, A. N., Lemak, S., Chernikova, T. N., Golyshina, O. V., Almendral, D., Ferrer, M., Golyshin, P. N. & Yakunin, A. F., 5 Jan 2025, In: Journal of Hazardous Materials. 481, p. 136540

Research output: Contribution to journal › Article › peer-review

Pressure adaptation is linked to thermal adaptation in salt-saturated marine habitats

Alcaide, M., Stogios, P. J., Lafraya, A., Tchigvintsev, A., Flick, R., Bargiela, R., Chernikova, T. N., Reva, O. N., Hai, T., Leggewie, C. C., Katzke, N., La Cono, V., Matesanz, R., Jebbar, M., Jaeger, K., Yakimov, M. M., Yakunin, A. F., Golyshin, P. N., Golyshina, O. V., Savchenko, A. & Ferrer, M., 17 Dec 2014, In: Environmental Microbiology. 17, 2, p. 332-345

Research output: Contribution to journal › Article › peer-review

Proteome cold-shock response in the extremely acidophilic archaeon, Cuniculiplasma divulgatum

Bargiela, R., Lanthaler, K., Potter, C., Ferrer, M., Yakunin, A., Paizs, B., Golyshin, P. & Golyshina, O., 19 May 2020, In: Microorganisms . 8, 5

Research output: Contribution to journal › Article › peer-review

Quantifying metal-binding specificity of CcNikZ-II from Clostridium carboxidivorans in the presence of competing metal ions

Diep, P., Kell, B., Yakunin, A., Hilfinger, A. & Mahadevan, R., 1 Sept 2023, In: Analytical biochemistry. 676, 115182.

Research output: Contribution to journal › Article › peer-review

Relationships between Substrate Promiscuity and Chiral Selectivity of Esterases from Phylogenetically and Environmentally Diverse Microorganisms

Coscolín, C., Martínez-Martínez, M., Chow, J., Bargiela, R., García-Moyano, A., Bjerga, G. E. K., Bollinger, A., Stokke, R., Steen, I. H., Golyshina, O., Yakimov, M. M., Jaeger, K.-E., Yakunin, A. F., Streit, W. R., Golyshin, P. & Ferrer, M., 5 Jan 2018, In: Catalysts. 8, 1, 10.

Research output: Contribution to journal › Article › peer-review

Screening and Characterization of Novel Polyesterases from Environmental Metagenomes with High Hydrolytic Activity against Synthetic Polyesters

Hajighasemi, M., Tchigvintsev, A., Nocek, B., Flick, R., Popovic, A., Hai, T., Khusnutdinova, A. N., Brown, G., Xu, X., Cui, H., Anstett, J., Chernikova, T., Bruls, T., Le Paslier, D., Yakimov, M. M., Joachimiak, A., Golyshina, O., Savchenko, A., Golyshin, P., Edwards, E. A. & Yakunin, A. F., 6 Nov 2018, In: Environmental Science and Technology. 52, 21, p. 12388-12401

Research output: Contribution to journal › Article › peer-review

Site-directed mutagenesis and stability of the carboxylic acid reductase MAB4714 from Mycobacterium abscessus

Fedorchuk, T. P., Khusnutdinova, A. N., Flick, R. & Yakunin, A., 10 Sept 2019, In: Journal of Biotechnology. 303, p. 72-79

Research output: Contribution to journal › Article › peer-review

Structural and biochemical insights into CRISPR RNA processing by the Cas5c ribonuclease SMU1763 from Streptococcus mutans

Lemak, S., Serbanescu, M. A., Khusnutdinova, A. N., Ruszkowski, M., Beloglazova, N., Xu, X., Brown, G., Cui, H., Tan, K., Joachimiak, A., Cvitkovitch, D. G., Savchenko, A. & Yakunin, A., Nov 2021, In: Journal of Biological Chemistry. 297, 5, 101251.

Research output: Contribution to journal › Article › peer-review

Structural insights into hydrolytic defluorination of difluoroacetate by microbial fluoroacetate dehalogenases

Khusnutdinova, A. N., Batyrova, K., Brown, G., Fedorchuck, T., Chai, Y. S., Skarina, T., Flick, R., Petit, A.-P., Savchenko, A., Stogios, P. & Yakunin, A., Oct 2023, In: Febs Journal. 290, 20, p. 4966-4983 18 p.

Research output: Contribution to journal › Article › peer-review

Structure and activity of the cold-active and anion-activated carboxyl esterase OLEI01171 from the oil-degrading marine bacterium Oleispira antarctica

Lemak, S., Tchigvintsev, A., Petit, P., Flick, R., Singer, A. U., Brown, G., Evdokimova, E., Egorova, O., Gonzalez, C. F., Chernikova, T. N., Yakimov, M. M., Kube, M., Reinhardt, R., Golyshin, P. N., Savchenko, A. & Yakunin, A. F., 15 Jul 2012, In: Biochemical Journal. 445, 2, p. 193-203

Research output: Contribution to journal › Article › peer-review

Systems engineering of Escherichia coli for n-butane production

Liu, Y., Khusnutdinova, A., Chen, J., Crisante, D., Batyrova, K., Raj, K., Feigis, M., Shirzadi, E., Wang, X., Dorakhan, R., Wang, X., Stogios, P. J., Yakunin, A. F., Sargent, E. H. & Mahadevan, R., 1 Nov 2022, In: Metabolic Engineering. 74, p. 98-107 10 p.

Research output: Contribution to journal › Article › peer-review

The environment shapes microbial enzymes: five cold-active and salt-resistant carboxylesterases from marine metagenomes

Tchigvintsev, A., Tran, H., Popvic, A., Kovacic, F., Brown, G., Flick, R., Hajighasemi, M., Egorova, O., Somody, J. C., Tchigvintsev, D., Khusnutdinova, A., Chernikova, T. N., Golyshina, O., Yakimov, M. M., Savchenko, A., Golyshin, P., Jaeger, K. E. & Yakunin, A. F., 1 Mar 2015, In: Applied Microbiology and Biotechnology. 99, 5, p. 2165-2178

Research output: Contribution to journal › Article › peer-review

Thermophilic Carboxylesterases from Hydrothermal Vents of the Volcanic Island of Ischia Active on Synthetic and Biobased Polymers and Mycotoxins

Distaso, M., Chernikova, T., Bargiela, R., Coscolín, C., Stogios, P. J., Gonzalez-Alfonso, J. L., Lemak, S., Khusnutdinova, A., Plou, F. J., Evdokimova, E., Savchenko, A., Lunev, E., Yakimov, M. M., Golyshina, O., Ferrer, M., Yakunin, A. & Golyshin, P., 28 Feb 2023, In: Applied and Environmental Microbiology. 89, 2, p. e0170422 20 p.

Research output: Contribution to journal › Article › peer-review

Harnessing extremophilic carboxylesterases for applications in polyester depolymerisation and plastic waste recycling

Williams, G., Ma, H., Khusnutdinova, A., Yakunin, A. & Golyshin, P., Aug 2023, In: Essays in Biochemistry. 67, 4, p. 715-729 15 p., EBC20220255.

Research output: Contribution to journal › Review article › peer-review