Novel metagenomic biocatalyst collections for industrial biotechnology MetaCat

  1. Article › Research › Peer-reviewed
  2. Published

    Activity screening of environmental metagenomic libraries reveals novel carboxylesterase families

    Popovic, A., Hai, T., Tchigvintsev, A., Hajighasemi, M., Nocek, B., Khusnutdinova, A. N., Brown, G., Glinos, J., Flick, R., Skarina, T., Chernikova, T., Yim, V., Bruls, T., Le Paslier, D., Yakimov, M. M., Joachimiak, A., Ferrer, M., Golyshina, O., Savchenko, A., Golyshin, P. & Yakunin, A. F., Mar 2017, In: Scientific Reports. 7, 44103, 44103.

    Research output: Contribution to journalArticlepeer-review

  3. Published

    Archaea dominate the microbial community in an ecosystem with low-to-moderate temperature and extreme acidity

    Korzhenkov, A. A., Toshchakov, S. V., Bargiela, R., Gibbard, H., Ferrer, M., Teplyuk, A. V., Jones, D. L., Kublanov, I. V., Golyshin, P. & Golyshina, O., 28 Jan 2019, In: Microbiome. 7, 1, 11.

    Research output: Contribution to journalArticlepeer-review

  4. Published

    Biodiversity for biocatalysis: A review of the α/β-hydrolase fold superfamily of esterases-lipases discovered in metagenomes

    Ferrer, M., Bargiela, R., Martínez-Martínez, M., Mir, J., Koch, R., Golyshina, O. & Golyshin, P., 1 Mar 2016, In: Biocatalysis and Biotransformation . 33, 5-6, p. 235-249

    Research output: Contribution to journalArticlepeer-review

  5. Published

    Biology of archaea from a novel family Cuniculiplasmataceae (Thermoplasmata) ubiquitous in hyperacidic environments

    Golyshina, O., Kublanov, I. V., Hai, T., Korzhenkov, A. A., Lunsdorf, H., Nechitaylo, T. Y., Toshchakov, S. V. & Golyshin, P., Dec 2016, In: Scientific Reports. 6, 39034.

    Research output: Contribution to journalArticlepeer-review

  6. Published

    Bioprospecting reveals class III ω-transaminases converting bulky ketones and environmentally relevant polyamines

    Coscolín, C., Katzke, N., Garcia-Moyano, A., Navarro-Fernández, J., Almendral, D., Martinez-Martinez, M., Bollinger, A., Bargiela, R., Gerler, C., Chernikova, T. N., Rojo, D., Barbas, C., Tran, H., Golyshina, O. V., Koch, R., Yakimov, M. M., Bjerga, G. E. K., Golyshin, P. N., Jaeger, K-E. & Ferrer, M., 9 Jan 2019, In: Applied and Environmental Microbiology. 85, 2, e02404-18.

    Research output: Contribution to journalArticlepeer-review

  7. Published

    Combined whole-cell high-throughput functional screening for identification of new nicotinamidases/pyrazinamidases in metagenomic/polygenomic libraries

    Zapata-Perez, R., Garcia-Saura, A. G., Jebbar, M., Golyshin, P. & Sanchez-Ferrer, A., Nov 2016, In: Frontiers in Microbiology. 7, 1915.

    Research output: Contribution to journalArticlepeer-review

  8. Published

    Cuniculiplasmataceae, their ecogenomic and metabolic patterns and interactions with ‘ARMAN’

    Golyshina, O. V., Bargiela, R. & Golyshin, P. N., Jan 2019, In: Extremophiles. 23, 1, p. 1-7 7 p.

    Research output: Contribution to journalArticlepeer-review

  9. Published

    Degradation Network Reconstruction in Uric Acid and Ammonium Amendments in Oil-Degrading Marine Microcosms Guided by Metagenomic Data

    Bargiela, R., Gertler, C., Mapelli, F., Magagnini, M., Chen, J., Daffonchio, D., Golyshin, P. & Ferrer, M., 24 Nov 2015, In: Frontiers in Microbiology. 6

    Research output: Contribution to journalArticlepeer-review

  10. Published

    Determinants and prediction of esterase substrate promiscuity patterns

    Martínez-Martínez, M., Coscolín, C., Santiago, G., Chow, J., Stogios, P. J., Bargiela, R., Gertler, C., Navarro-Fernández, J., Bollinger, A., Thies, S., Méndez-García, C., Popovic, A., Brown, G., Chernikova, T., García-Moyano, A., Bjerga, G. E. K., Perez-Garcia, P., Hai, T., del Pozo, M. V., Stokke, R., Steen, I. H., Cui, H., Xu, X., Nocek, B., Alcaide, M., Disasto, M., Mesa, V., Pelaez, A. I., Sanchez, J., Buchholz, P. C. F., Pleiss, J., Fernández-Guerra, A., Glockner, F. O., Golyshina, O., Yakimov, M. M., Savchenko, A., Jaeger, K-E., Yakunin, A. F., Streit, W. R., Golyshin, P., Guallar, V. & Ferrer, M., 2018, In: ACS Chemical Biology. 13, 1, p. 225-234

    Research output: Contribution to journalArticlepeer-review

  11. Published

    Diversity of “Ca. Micrarchaeota” in two distinct types of acidic environments and their associations with Thermoplasmatales

    Golyshina, O., Bargiela, R., Toshchakov, S. V., Chernyh, N. A., Ramayah, S., Korzhenkov, A. A., Kublanov, I. V. & Golyshin, P., 15 Jun 2019, In: Genes. 10, 6, 461.

    Research output: Contribution to journalArticlepeer-review

  12. Published

    Estimating the success of enzyme bioprospecting through metagenomics: current status and future trends

    Ferrer, M., Martinez, M., Bargiela, R., Streit, W. R., Golyshina, O. & Golyshin, P., 1 Jan 2016, In: Microbial Biotechnology. 9, 1, p. 22-34

    Research output: Contribution to journalArticlepeer-review

  13. Published

    Genetically engineered proteins with two active sites for enhanced biocatalysis and synergistic chemo- and biocatalysis

    Alonso, S., Santiago, G., Cea-Rama, I., Fernandez-Lopez, L., Coscolín, C., Modregger, J., Ressmann, A. K., Martinez-Martinez, M., Marrero, H., Bargiela, R., Pita, M., Gonzalez-Alfonso, J. L., Briand, M. L., Rojo, D., Barbas, C., Plou, F. J., Golyshin, P., Shahgaldian, P., Sanz-Aparicio, J., Guallar, V. & Ferrer, M., Mar 2020, In: Nature Catalysis. 3, 3, p. 319-328

    Research output: Contribution to journalArticlepeer-review

  14. Published

    Metabolic and evolutionary patterns in the extremely acidophilic archaeon Ferroplasma acidiphilum YT

    Golyshina, O., Hai, T., Reva, O. N., Lemak, S., Yakunin, A. F., Goesmann, A., Nechitaylo, T. Y., LaCono, V., Smedile, F., Slesarev, A., Rojo, D., Barbas, C., Ferrer, M., Yakimov, M. M. & Golyshin, P., 16 Jun 2017, In: Scientific Reports. 7, 3682.

    Research output: Contribution to journalArticlepeer-review

  15. Published

    Microbial Communities of Polymetallic Deposits’ Acidic Ecosystems of Continental Climatic Zone With High Temperature Contrasts

    Gavrilov, S. N., Korzhenkov, A. A., Kublanov, I. V., Bargiela, R., Zamana, L. V., Toshchakov, S. V., Golyshin, P. & Golyshina, O., 17 Jul 2019, In: Frontiers in Microbiology.

    Research output: Contribution to journalArticlepeer-review

  16. Published

    Proteogenomic Analysis of Epibacterium Mobile BBCC367, a Relevant Marine Bacterium Isolated From the South Pacific Ocean

    Matallana-Surget, S., Werner, J., Wattiez, R., Lebaron, K., Intertaglia, L., Regan, C., Morris, J., Teeling, H., Ferrer, M., Golyshin, P., Gerogiorgis, D., Reilly, S. I. & Lebaron, P., Dec 2018, In: Frontiers in Microbiology. 9, 3125.

    Research output: Contribution to journalArticlepeer-review

  17. Published

    Rational Engineering of Multiple Active Sites in an Ester Hydrolase

    Santiago, G., Martinez-Martinez, M., Alonso, S., Bargiela, R., Coscolín, C., Golyshin, P., Guallar, V. & Ferrer, M., Mar 2018, In: Biochemistry. 57, 15, p. 2245-2255

    Research output: Contribution to journalArticlepeer-review

  18. Published

    Relationships between Substrate Promiscuity and Chiral Selectivity of Esterases from Phylogenetically and Environmentally Diverse Microorganisms

    Coscolín, C., Martínez-Martínez, M., Chow, J., Bargiela, R., García-Moyano, A., Bjerga, G. E. K., Bollinger, A., Stokke, R., Steen, I. H., Golyshina, O., Yakimov, M. M., Jaeger, K-E., Yakunin, A. F., Streit, W. R., Golyshin, P. & Ferrer, M., 5 Jan 2018, In: Catalysts. 8, 1, 10.

    Research output: Contribution to journalArticlepeer-review

  19. Published

    Screening and Characterization of Novel Polyesterases from Environmental Metagenomes with High Hydrolytic Activity against Synthetic Polyesters

    Hajighasemi, M., Tchigvintsev, A., Nocek, B., Flick, R., Popovic, A., Hai, T., Khusnutdinova, A. N., Brown, G., Xu, X., Cui, H., Anstett, J., Chernikova, T., Bruls, T., Le Paslier, D., Yakimov, M. M., Joachimiak, A., Golyshina, O., Savchenko, A., Golyshin, P., Edwards, E. A. & Yakunin, A. F., 6 Nov 2018, In: Environmental Science and Technology. 52, 21, p. 12388-12401

    Research output: Contribution to journalArticlepeer-review

  20. Published

    Structure and evolutionary trace-assisted screening of a residue swapping the substrate ambiguity and chiral specificity in an esterase

    Cea-Rama, I., Coscolín, C., Katsonis, P., Bargiela, R., Golyshin, P., Lichtarge, O., Ferrer, M. & Sanz-Aparicio, J., 2021, In: Computational and Structural Biotechnology Journal. 19, p. 2307-2317 11 p.

    Research output: Contribution to journalArticlepeer-review

  21. Published

    Subtle shifts in microbial communities occur alongside the release of carbon induced by drought and rewetting in contrasting peatland ecosystems

    Potter, C., Freeman, C., Golyshin, P., Ackerman, G., Fenner, N., McDonald, J., Ehbair, A., Jones, T., Murphy, L. & Creer, S., Sept 2017, In: Scientific Reports. 7, 11314.

    Research output: Contribution to journalArticlepeer-review

  22. Published

    Sulfur Respiration in a Group of Facultatively Anaerobic Natronoarchaea Ubiquitous in Hypersaline Soda Lakes

    Sorokin, D. Y., Messina, E., La Cono, V., Ferrero, M., Ciordia, S., Mena, M. C., Toshchakov, S. V., Golyshin, P. N. & Yakimoy, M. M., Oct 2018, In: Frontiers in Microbiology. 9, 2359.

    Research output: Contribution to journalArticlepeer-review

  23. Published

    The genome analysis of Oleiphilus messinensis ME102 (DSM 13489T) reveals backgrounds of its obligate alkane-devouring marine lifestyle

    Toshchakov, S. V., Korzhenkov, A. A., Chernikova, T., Ferrer, M., Golyshina, O., Yakimov, M. M. & Golyshin, P., Dec 2017, In: Marine Genomics. 36, p. 41-47

    Research output: Contribution to journalArticlepeer-review

  24. Published

    The novel extremely acidophilic, cell-wall-deficient archaeon Cuniculiplasma divulgatum gen. nov., sp. nov. represents a new family, Cuniculiplasmataceae fam. nov., of the order Thermoplasmatales

    Golyshina, O., Lünsdorf, H., Kublanov, I. V., Goldenstein, N. I., Hinrichs, K-U. & Golyshin, P., Jan 2016, In: International Journal of Systematic and Evolutionary Microbiology. 66, 1, p. 332-340

    Research output: Contribution to journalArticlepeer-review

  25. Published

    The rumen microbiome: an underexplored resource for novel antimicrobial discovery

    Oyama, L. B., Girdwood, S. E., Cookson, A. R., Fernanadez-Fuentes, N., Prive, F., Vallin, H. E., Wilkinson, T. J., Golyshin, P., Golyshina, O., Mikut, R., Hilpert, K., Richards, J., Wootton, M., Edwards, J. E., Maresca, M., Perrier, J., Lundy, F. T., Luo, Y., Zhou, M., Hess, M., Mantovani, H. C., Creevey, C. J. & Huws, S. A., 1 Dec 2017, In: NPJ Biofilms and Microbiomes. 3, 33 .

    Research output: Contribution to journalArticlepeer-review

  26. Published

    “ARMAN” archaea depend on association with euryarchaeal host in culture and in situ

    Golyshina, O., Toshchakov, S. V., Makarova, K. S., Gavrilov, S. N., Korzhenkov, A. A., La Cono, V., Arcadi, E., Nechitaylo, T. Y., Ferrer, M., Kublanov, I. V., Wolf, Y. I., Yakimov, M. M. & Golyshin, P., 2017, In: Nature Communications. 8, 60.

    Research output: Contribution to journalArticlepeer-review